CAJ | 학술논문

采用菌丝生长速率法测定了中药植物金线吊乌龟块根甲醇提取物对5种植物病原真菌的抗菌活性,结果表明:金线吊乌龟块根提取物对梨锈病菌、稻瘟病菌、水稻纹枯病菌和玉米炭疽病菌有很高的抑制活性,质量浓度为10 g/L时,抑菌率分别为97.74%、100.00%、94.51%和84.40%;有效中浓度(EC_(50))分别为0.587 9、0.312 2、0.280 7 g/L和0.835 3 g/L.对柿角斑病菌的活性较低,在10 g/L质量浓度下,抑菌率只有67.59%,EC_(50)为1.349 1 g/L.金线吊乌龟块根提取物对甘蔗凤梨病菌孢子、柑橘疮痂病菌孢子和梨锈病菌孢子的萌发具有明显的抑制活性,质量浓度为10 g/L时,抑制率分别为91.80%、97.00%和100.00%;对西瓜枯萎病菌孢子萌发的抑制效果比较差,抑制率仅为20.63%.按生物碱分离程序对金线吊乌龟块根提取物进行初步分离,结合活性跟踪,发现氯仿层生物碱是金线吊乌龟的主要抗菌活性成分.
채용균사생장속솔법측정료중약식물금선조오구괴근갑순제취물대5충식물병원진균적항균활성,결과표명:금선조오구괴근제취물대리수병균、도온병균、수도문고병균화옥미탄저병균유흔고적억제활성,질량농도위10 g/L시,억균솔분별위97.74%、100.00%、94.51%화84.40%;유효중농도(EC_(50))분별위0.587 9、0.312 2、0.280 7 g/L화0.835 3 g/L.대시각반병균적활성교저,재10 g/L질량농도하,억균솔지유67.59%,EC_(50)위1.349 1 g/L.금선조오구괴근제취물대감자봉리병균포자、감귤창가병균포자화리수병균포자적맹발구유명현적억제활성,질량농도위10 g/L시,억제솔분별위91.80%、97.00%화100.00%;대서과고위병균포자맹발적억제효과비교차,억제솔부위20.63%.안생물감분리정서대금선조오구괴근제취물진행초보분리,결합활성근종,발현록방층생물감시금선조오구적주요항균활성성분.
The antifungal activity of the methanol extract from the tuberous root of Stephania cepharantha Hayata against 5 kinds of plant pathogenic fungi was determined using mycelium growth rate method. The results showed that the extract from the tuberous root of S. Cepharantha had high inhibitory activity against Gymnosporangium haraeanum Syd., Pyricularia oryzae Cav., Rhizoctonia solani Kuhn, and Colletotrichum graminicola (Ces.) Wil-son., with the inhibition rates of 97.74%, 100.00%, 94.51% and 84.40% at 10 g/L, respectively, and the EC_(50) values of 0.587 9g/L, 0.312 2 g/L, 0.280 7 g/L and 0.835 3 g/L, respectively. The antifungal activity of the extract against Cercospora kaki Ell. Et Ev. Was weaker, with the inhibition rate of 67.59% at 10 g/L, and the EC_(50) value of 1.349 1 g/L. The extract of the tuberous root of S. Cepharantha also had significant inhibitory ac-tivity against spore germination of Thielaviopsis paradoxa (de Seynes) V. Hohnel, Sphaceloma fawcettii Jenkins and G. Haraeanum Syd., with the inhibition rates of 91.80%, 97.00% and 100.00% at 10 g/L, respectively. The inhibitory activity against spore germination of Fusarium oxysporum f. Sp. Niveum (E. F. Smith) Syn. Et Hans was weaker, with the inhibition rate of 20.63% at 10g/L. The methanol extract from the tuberous root of S. Cepharantha was preliminarily separated into 3 parts by the method of alkaloid isolation, and the results of bioas-say showed that the active ingredients mainly consisted in the chloroform fraction.