CAJ | 학술논문

目的研究血管紧张素受体Ⅱ(ATⅡ)的AT1受体阻滞剂伊贝沙坦对2型糖尿病(T2DM)大鼠肾皮质趋化因子fractalkine表达的影响.方法制备T2DM大鼠模型,随机分为正常对照组、糖尿病组及伊贝沙坦治疗组.分别用半定量RT-PCR和免疫组化法检测肾皮质fractalkine mRNA和蛋白表达.结果糖尿病组血清糖基化终产物-肽、尿素氮(BUN)、24h尿总蛋白(24hUPro)、肾皮质fractalkine mRNA 和蛋白表达均明显高于对照组;伊贝沙坦治疗组血清BUN、24hUPro、肾皮质fractalkine mRNA和蛋白表达则明显低于糖尿病组.结论伊贝沙坦可能通过抑制肾皮质fractalkine表达发挥其肾保护作用.
목적 연구혈관긴장소수체Ⅱ(ATⅡ)적AT1수체조체제이패사탄대2형당뇨병(T2DM)대서신피질추화인자fractalkine표체적영향.방법 제비T2DM대서모형,수궤분위정상대조조、당뇨병조급이패사탄치료조.분별용반정량RT-PCR화면역조화법검측신피질fractalkine mRNA화단백표체.결과당뇨병조혈청당기화종산물-태、뇨소담(BUN)、24h뇨총단백(24hUPro)、신피질fractalkine mRNA 화단백표체균명현고우대조조;이패사탄치료조혈청BUN、24hUPro、신피질fractalkine mRNA화단백표체칙명현저우당뇨병조.결론 이패사탄가능통과억제신피질fractalkine표체발휘기신보호작용.
Objective To investigate the effect of irbesartan on the expression of fractalkine in renal cortex of type 2 diabetic rats. Methods Type 2 diabetic rats were induced by an intraperitoneal injection of low doses of streptozotocin combined with high-fat diet， and were randomized to receive irbesartan or vehicle treatment.The mRNA and protein levels of fractalkine were detected by reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemical staining， respectively. Results AGE-P, serum BUN, 24hUPro，the levels of fractalkine mRNA and protein in renal cortex were significantly higher in diabetic rats than in normal control. However, those of rats treated with irbesartan were obviously lower as compared to untreated group. Conclusions Irbesartan may play the renoprotective role via inhibition of fractalkine expression in the renal cortex.