CAJ | 학술논문

目的探讨内毒索受体CD14-1159C/T基因多态性与术后严重感染易感性及预后的关系.方法以收治的42例术后严重感染患者为对象,以正常献血者为对照,提取全血基因组DNA,应用限制性长度片段多态性(RFLP)方法检测CD14-159C/T基因多态性,并研究CD14基因多态性与术后严重感染易感性及病死率的关系.结果 42例术后严重感染患者的T等位基因频率(78.4%)明显高于C等位基因频率(21.6%),且-159TT基因型患者病死率(84.6%)明显高于-159CT(15.4%)及-159CC患者(0)(P＜0.05).结论 CD14-159C/T基因多态性与术后严重感染的易感性及预后有一定关系.
목적 탐토내독색수체CD14-1159C/T기인다태성여술후엄중감염역감성급예후적관계.방법 이수치적42례술후엄중감염환자위대상,이정상헌혈자위대조,제취전혈기인조DNA,응용한제성장도편단다태성(RFLP)방법검측CD14-159C/T기인다태성,병연구CD14기인다태성여술후엄중감염역감성급병사솔적관계.결과 42례술후엄중감염환자적T등위기인빈솔(78.4%)명현고우C등위기인빈솔(21.6%),차-159TT기인형환자병사솔(84.6%)명현고우-159CT(15.4%)급-159CC환자(0)(P＜0.05).결론 CD14-159C/T기인다태성여술후엄중감염적역감성급예후유일정관계.
Objective To investigate the relation of a lipopolysaccharide receptor CD14C-159T gene polymorphism to severe sepsis susceptibility and outcome in postoperation.Methods A prospective,consecutive entry study WSS made among 42 postoperative severe sepsis admitted in neurosurgeon,and 50 health volunteers.The genomic DNA of peripheral blood nucleated was extracted.Typing of each patient for the CD14-159C/T gene polymorphism was performed by restrictive fragment length polymorphism(RFLP).Genotypes were then related to the susceptibility and mortality rate of severe sepsis.Results Development of postoperative severe sepsis increased in patients homozygous for the allele T(78.4%)than that of the allele C(21.6%)(P＜0.05).The mortalitv of sepsis were significantly higher in patients with CD14 homozygous for the T allete(TT)(84.6%)than those with genotype TC (15.4%)and C ailetc(CC)(O).Conclusion The single base pair polymorphism at position-159 in the CD14 gene promoter might influence the development of severe sepsis as well as outcome in postopration.