国际中医中药杂志
國際中醫中藥雜誌
국제중의중약잡지
INTERNATIONAL JOURNAL OF TRIDITIONAL CHINESE MEDICINE
2011年
4期
313-315
,共3页
复方阿胶浆%移植性lewis肺癌%VEGF
複方阿膠漿%移植性lewis肺癌%VEGF
복방아효장%이식성lewis폐암%VEGF
Compound E-Jiao slurry%Lewis lung carcinoma xenografts%VEGF
目的 观察复方阿胶浆联合化疗对移植性lewis肺癌组织血管内皮生长因子(VEGF)表达的影响.方法 用清洁级雄性C57BL/6小鼠50只制作lewis肺癌移植瘤模型,按体重将小鼠随机分为模型对照组、环磷酰胺组、复方阿胶浆组、复方阿胶浆大剂量联合环磷酰胺(联合用药1)、复方阿胶浆中剂量联合环磷酰胺(联合用药2)5组,每组10只.给药结束后,取瘤组织石蜡包埋、切片,HE染色,光镜下观察组织形态;取上述5组切片以免疫组化染色法观察VEGF蛋白表达.结果 环磷酰胺、联合用药1、2组较模型对照组VEGF表达明显下降,平均光密度值分别为(0.1959±0.0233)、(0.1921±0.0218)、(0.1988±0.0432)、(0.2612±0.0219),(P＜0.05);复方阿胶浆组与模型对照组比较,平均光密度为(0.2496±0.0298),差异无统计学意义(P＞0.05);联合用药1、2组与环磷酰胺组比较,差异无统计学意义(P＞0.05).结论 复方阿胶浆对肺癌组织VEGF表达无明显作用,伍用化疗后不会影响化疗药物对VEGF表达的下调作用.
目的 觀察複方阿膠漿聯閤化療對移植性lewis肺癌組織血管內皮生長因子(VEGF)錶達的影響.方法 用清潔級雄性C57BL/6小鼠50隻製作lewis肺癌移植瘤模型,按體重將小鼠隨機分為模型對照組、環燐酰胺組、複方阿膠漿組、複方阿膠漿大劑量聯閤環燐酰胺(聯閤用藥1)、複方阿膠漿中劑量聯閤環燐酰胺(聯閤用藥2)5組,每組10隻.給藥結束後,取瘤組織石蠟包埋、切片,HE染色,光鏡下觀察組織形態;取上述5組切片以免疫組化染色法觀察VEGF蛋白錶達.結果 環燐酰胺、聯閤用藥1、2組較模型對照組VEGF錶達明顯下降,平均光密度值分彆為(0.1959±0.0233)、(0.1921±0.0218)、(0.1988±0.0432)、(0.2612±0.0219),(P＜0.05);複方阿膠漿組與模型對照組比較,平均光密度為(0.2496±0.0298),差異無統計學意義(P＞0.05);聯閤用藥1、2組與環燐酰胺組比較,差異無統計學意義(P＞0.05).結論 複方阿膠漿對肺癌組織VEGF錶達無明顯作用,伍用化療後不會影響化療藥物對VEGF錶達的下調作用.
목적 관찰복방아효장연합화료대이식성lewis폐암조직혈관내피생장인자(VEGF)표체적영향.방법 용청길급웅성C57BL/6소서50지제작lewis폐암이식류모형,안체중장소서수궤분위모형대조조、배린선알조、복방아효장조、복방아효장대제량연합배린선알(연합용약1)、복방아효장중제량연합배린선알(연합용약2)5조,매조10지.급약결속후,취류조직석사포매、절편,HE염색,광경하관찰조직형태;취상술5조절편이면역조화염색법관찰VEGF단백표체.결과 배린선알、연합용약1、2조교모형대조조VEGF표체명현하강,평균광밀도치분별위(0.1959±0.0233)、(0.1921±0.0218)、(0.1988±0.0432)、(0.2612±0.0219),(P＜0.05);복방아효장조여모형대조조비교,평균광밀도위(0.2496±0.0298),차이무통계학의의(P＞0.05);연합용약1、2조여배린선알조비교,차이무통계학의의(P＞0.05).결론 복방아효장대폐암조직VEGF표체무명현작용,오용화료후불회영향화료약물대VEGF표체적하조작용.
Objective To observe the effects of CEJS combined with chemotherapy on the expression of VEGF in lewis lung carcinoma.Methods Tumor xenografts models were set up and randomly divided into five groups including control group,CTX CEJS,high-dose CEJS joint CTX(combination group 1),and middle-dose CEJS joint CTX(combination group 2).Paraffin imbedding and HE staining were performed,and the tissue Shape was observed by microscope after section.The expressions of VEGF in five groups were determined by immuno-histochemistry.Results The expression level of VEGF could be significantly down-regulated in CTX or combination group 1 and 2,compared with the control group(P＜0.05):CEJS group had no significant difference to the control group(P＞0.05);The average optical density in the combination group 1 and 2 showed no significantly difference to the CTX group(P＞0.05).Conclusion The inhibition of CEJS could not down regulate of the expression of VEGF,but CEJS could keep the affection of chemotherapy drugs on VEGF.
