CAJ | 학술논문

目的探讨膀胱尿路上皮癌组织中凋亡抑制因子Livin和血管内皮生长因子(VEGF)蛋白的表达与临床病理参数的关系及其相关性.方法采用免疫组织化学S-P法检测69例膀胱尿路上皮癌和10例正常膀胱黏膜组织中Livin和VEGF蛋白的表达状况,结合临床病理学资料进行统计学分析.结果 Livin和VEGF蛋白在膀胱尿路上皮癌组织的阳性表达率分别为65.2%(45/69)和46.4%(32/69),而正常膀胱黏膜组织中均不表达(均为0.0%),组间比较差异有统计学意义(P均＜0.01).在有、无复发组的膀胱尿路上皮癌组织中Livin蛋白的阳性表达率差异有统计学意义[有复发78.8%(26/33),无复发48.1%(13/27),χ2=6.13,P＜0.05],而在不同病理分级组、不同临床分期组及肿瘤单、多发组的膀胱尿路上皮癌组织中Livin蛋白的阳性表达率差异均无统计学意义(G1 55.6%,G2 64.3%,G3 73.9%;Ta～T1 61.9%,T2～T4 70.4%;单发59.6%,多发77.3%;χ2值分别为1.52、0.52、2.07,P均＞0.05).在不同病理分级组和不同临床分期组的膀胱尿路上皮癌组织中VEGF蛋白的阳性表达率差异均有统计学意义(G116.7%,G2 53.6%,G3 60.9%;Ta～T1 33.3%,T2～T4 66.7%;χ2值分别为8.91、7.34,P＜0.05或P＜0.01),而在肿瘤单、多发组和有、无复发组的膀胱尿路上皮癌组织中VEGF蛋白的阳性表达率差异均无统计学意义(单发46.8%,多发45.5%;有复发51.5%,无复发40.7%,χ2值分别为0.01、0.69,P均＞0.05).膀胱尿路上皮癌组织中Livin与VEGF蛋白的表达无相关关系(r=0.056,P＞0.05).结论 Livin和VEGF蛋白的高表达可能在膀胱尿路上皮癌的发生过程中起重要作用.联合检测二者的变化有望成为膀胱尿路上皮癌诊断和判断预后的一项客观指标.
목적 탐토방광뇨로상피암조직중조망억제인자Livin화혈관내피생장인자(VEGF)단백적표체여림상병리삼수적관계급기상관성.방법 채용면역조직화학S-P법검측69례방광뇨로상피암화10례정상방광점막조직중Livin화VEGF단백적표체상황,결합림상병이학자료진행통계학분석.결과 Livin화VEGF단백재방광뇨로상피암조직적양성표체솔분별위65.2%(45/69)화46.4%(32/69),이정상방광점막조직중균불표체(균위0.0%),조간비교차이유통계학의의(P균＜0.01).재유、무복발조적방광뇨로상피암조직중Livin단백적양성표체솔차이유통계학의의[유복발78.8%(26/33),무복발48.1%(13/27),χ2=6.13,P＜0.05],이재불동병리분급조、불동림상분기조급종류단、다발조적방광뇨로상피암조직중Livin단백적양성표체솔차이균무통계학의의(G1 55.6%,G2 64.3%,G3 73.9%;Ta～T1 61.9%,T2～T4 70.4%;단발59.6%,다발77.3%;χ2치분별위1.52、0.52、2.07,P균＞0.05).재불동병리분급조화불동림상분기조적방광뇨로상피암조직중VEGF단백적양성표체솔차이균유통계학의의(G116.7%,G2 53.6%,G3 60.9%;Ta～T1 33.3%,T2～T4 66.7%;χ2치분별위8.91、7.34,P＜0.05혹P＜0.01),이재종류단、다발조화유、무복발조적방광뇨로상피암조직중VEGF단백적양성표체솔차이균무통계학의의(단발46.8%,다발45.5%;유복발51.5%,무복발40.7%,χ2치분별위0.01、0.69,P균＞0.05).방광뇨로상피암조직중Livin여VEGF단백적표체무상관관계(r=0.056,P＞0.05).결론 Livin화VEGF단백적고표체가능재방광뇨로상피암적발생과정중기중요작용.연합검측이자적변화유망성위방광뇨로상피암진단화판단예후적일항객관지표.
Objective To investigate the expressions of Livin protein and VEGF protein in bladder urothelial carcinoma(BUC) ,and theirs relationships with the clinicopathologic parameters of bladder urothelial carcinoma. Methods The expression of Livin and VEGF protein in 69 samples of BUC tissue and 10 samples of normal bladder epithelium tissue were detected by immunohistochemical SP method. Their relationships with clinicopathologic data were statistically analyzed. Results The positive expression rate of Livin and VEGF in BUC tissues were 65.2% (45/69) and 46.4% ( 32/69), but negative in normal bladder epithelium tissues, which showed significant differences in the comparison (Ps ＜ 0. 05 ). We found significant difference in the comparison of Livin positive rate between groups with or without recurrence ( 78. 8 % vs 48. 1%, χ2 = 6. 13, P ＜ 0. 05 ); but no differences in pathological grade,TNM stage and tumor number( Gl 55.6% ,G2 64. 3% ,G3 73.9% ;Ta ～ T1 61.9% ,T2 ～ T4 70. 4%; Single-tumor 59. 6%, multi-tumor 77.3%; χ2 = 1.52,0. 52,2.07, Ps ＞ 0. 05 ). For BUC,the expression of VEGF was correlated with the pathological grade,TNM stage( Gl 16. 7% ,G2 53.6% ,G3 60. 9%, χ2 = 8. 91; Ta ～ T1 33.3%, T2 ～ T4 66. 7%; χ2 = 7. 34; Ps ＜ 0. 05 ), but not the tumor number and recurrence( Single-tumor 57.4% , multi-tumor 59. 1%, χ2 = 0. 01; with recurrence 51.5% , without recurrence 40. 7% ,χ2= 0. 69; Ps ＞ 0. 05 ). We found no relationship between the expression of Livin and VEGF (r =0. 056,P ＞ 0. 05 ). Conclusion The overexpressions of Livin and VEGF protein may play an important role in the occurrence and development of BUC. Combind detection of these two protein can be used in the diagnosis and prognosis of BUC.