中国危重病急救医学
中國危重病急救醫學
중국위중병급구의학
CHINESE CRITICAL CARE MEDICINE
2009年
2期
79-81
,共3页
崔速南%汪明明%刘靓雯%张照华%于淑丽
崔速南%汪明明%劉靚雯%張照華%于淑麗
최속남%왕명명%류정문%장조화%우숙려
肝炎,乙型%穿孔素%颗粒酶%肝炎病毒,乙型%脱氧核糖核酸%流式细胞仪
肝炎,乙型%穿孔素%顆粒酶%肝炎病毒,乙型%脫氧覈糖覈痠%流式細胞儀
간염,을형%천공소%과립매%간염병독,을형%탈양핵당핵산%류식세포의
hepatitis B%perforin%granzyme%hepatitis B virus%DNA%flow cytometry
目的 探讨外周血淋巴细胞穿孔素/颗粒酶的表达在重型乙型肝炎(乙肝)肝细胞损伤中的作用,及其与乙型肝炎病毒(HBV)-DNA载量的关系.方法 取38例重型乙肝患者入院次日晨空腹静脉血备检;同期选择20例成年健康体检志愿者作为健康对照组;采用流式细胞仪测定外周血淋巴细胞穿孔素/颗粒酶的表达;采用荧光定量聚合酶链反应(PCR)测定血清HBV-DNA载量.结果 重型肝炎组外周血淋巴细胞穿孔素及颗粒酶阳性率均较健康对照组显著升高[穿孔素:(43.42±19.28)%比(19.65±9.27)%,颗粒酶:(40.35±12.26)%比(22.28±9.35)%,P均＜0.01],且血清HBV-DNA载量越高,重型乙肝患者外周血淋巴细胞穿孔素/颗粒酶表达强度越低,均呈明显负相关(r 孔素=0.92,r颗料酶=-0.96,P均＜0.01).结论 外周血淋巴细胞穿孔素/颗粒酶的过度表达是造成重型肝炎肝细胞损伤的重要因素,这种表达作用与乙型肝炎患者的HBV-DNA清除有关.
目的 探討外週血淋巴細胞穿孔素/顆粒酶的錶達在重型乙型肝炎(乙肝)肝細胞損傷中的作用,及其與乙型肝炎病毒(HBV)-DNA載量的關繫.方法 取38例重型乙肝患者入院次日晨空腹靜脈血備檢;同期選擇20例成年健康體檢誌願者作為健康對照組;採用流式細胞儀測定外週血淋巴細胞穿孔素/顆粒酶的錶達;採用熒光定量聚閤酶鏈反應(PCR)測定血清HBV-DNA載量.結果 重型肝炎組外週血淋巴細胞穿孔素及顆粒酶暘性率均較健康對照組顯著升高[穿孔素:(43.42±19.28)%比(19.65±9.27)%,顆粒酶:(40.35±12.26)%比(22.28±9.35)%,P均＜0.01],且血清HBV-DNA載量越高,重型乙肝患者外週血淋巴細胞穿孔素/顆粒酶錶達彊度越低,均呈明顯負相關(r 孔素=0.92,r顆料酶=-0.96,P均＜0.01).結論 外週血淋巴細胞穿孔素/顆粒酶的過度錶達是造成重型肝炎肝細胞損傷的重要因素,這種錶達作用與乙型肝炎患者的HBV-DNA清除有關.
목적 탐토외주혈림파세포천공소/과립매적표체재중형을형간염(을간)간세포손상중적작용,급기여을형간염병독(HBV)-DNA재량적관계.방법 취38례중형을간환자입원차일신공복정맥혈비검;동기선택20례성년건강체검지원자작위건강대조조;채용류식세포의측정외주혈림파세포천공소/과립매적표체;채용형광정량취합매련반응(PCR)측정혈청HBV-DNA재량.결과 중형간염조외주혈림파세포천공소급과립매양성솔균교건강대조조현저승고[천공소:(43.42±19.28)%비(19.65±9.27)%,과립매:(40.35±12.26)%비(22.28±9.35)%,P균＜0.01],차혈청HBV-DNA재량월고,중형을간환자외주혈림파세포천공소/과립매표체강도월저,균정명현부상관(r 공소=0.92,r과료매=-0.96,P균＜0.01).결론 외주혈림파세포천공소/과립매적과도표체시조성중형간염간세포손상적중요인소,저충표체작용여을형간염환자적HBV-DNA청제유관.
Objective To explore role of perforin/granzyme expression of peripheral blood lymphocyte in injury of hepatocyte in severe hepatitis B,and evaluate relationship between perforin/granzyme expression levels and hepatitis B virus(HBV)-DNA load.Methods Thirty eight patients of severe hepatitis B were enrolled in the study.Fasting venous blood was collected on following morning of admission.Twenty adult healthy subjects served as healthy control group.Perforin/granzyme expression of peripheral blood lymphocyte was detected by flow eytometry,and serum HBV-DNA load was detected by fluorescence quantitative polymerase chain reaction(PCR).Results Positive rate of perforin/granzyme in peripheral blood lymphocyte in severe hepatitis B was higher than that of the healthy control group[perforin:(43.42±19.28)%vs.(19.65±9.27)%,granzyme:(40.35±12.26)%vs.(22.28±9.35)%,both P＜0.01].There was a significant negative correlation between the perforin/granzyme expression of peripheral blood lymphocyte and serum HBV-DNA load(perforin=-0.92,raranzyme=-0.96,both P＜0.01),the higher serumHBV-DNA load,the lower perforin/granzyme expression in severe hepatitis B.Conclusion Perforin/granzyme overexpression in peripheral blood lymphocyte is an important factor in injury of hepatocyte in patients with severe hepatitis B,and the expression may be involved in HBV-DNA cleanup.
