CAJ | 학술논문

目的观察脓毒症时有核细胞过氧化物酶体增殖物激活受体γ(PPAR7)活性、血浆促炎介质白细胞介索-6(IL-6)的变化,探讨两者的关系.方法按随机数字表法将90只SD大鼠分为正常对照组、假手术组、脓毒症组,每组再分为术后12、24、48 h 3个亚组,每组10只.采用盲肠结扎穿孔术(CLP)制作大鼠脓毒症模型,用酶联免疫吸附法(ELISA)测定有核细胞PPAR7活性及血浆IL-6水平.结果脓毒症组术后12、24、48 h有核细胞PPART活性(A值:0.279±60.004、0.264±0.009、0.245±0.012)较正常对照组(0.292±0.007、0.293±0.004、0.293±0.005)和假手术组(0.295±0.008、0.295±0.006、0.294±0.007)明显下降,而血浆IL-6水平(ng/L:365.25±15.53、507.16±20.86、437.89±25.09)较正常对照组(43.54±11.10、48.82±10.62、42.96±9.52)和假手术组(42.43±6.77、40.32±6.48、44.10±9.36)明显升高(均P＜0.05);且脓毒症组随病情加重PPARγ活性逐渐下降,IL-6于24 h达高峰后下降,组内各时间点间两两比较差异均有统计学意义(均P＜0.05).脓毒症大鼠12 h时有核细胞PPARγ活性与血浆IL-6水平呈显著负相关(r=-0.703,P=0.023).结论脓毒症有核细胞PPARγ活性下降,血浆促炎介质IL-6升高,且两者呈负相关.
목적 관찰농독증시유핵세포과양화물매체증식물격활수체γ(PPAR7)활성、혈장촉염개질백세포개색-6(IL-6)적변화,탐토량자적관계.방법 안수궤수자표법장90지SD대서분위정상대조조、가수술조、농독증조,매조재분위술후12、24、48 h 3개아조,매조10지.채용맹장결찰천공술(CLP)제작대서농독증모형,용매련면역흡부법(ELISA)측정유핵세포PPAR7활성급혈장IL-6수평.결과 농독증조술후12、24、48 h유핵세포PPART활성(A치:0.279±60.004、0.264±0.009、0.245±0.012)교정상대조조(0.292±0.007、0.293±0.004、0.293±0.005)화가수술조(0.295±0.008、0.295±0.006、0.294±0.007)명현하강,이혈장IL-6수평(ng/L:365.25±15.53、507.16±20.86、437.89±25.09)교정상대조조(43.54±11.10、48.82±10.62、42.96±9.52)화가수술조(42.43±6.77、40.32±6.48、44.10±9.36)명현승고(균P＜0.05);차농독증조수병정가중PPARγ활성축점하강,IL-6우24 h체고봉후하강,조내각시간점간량량비교차이균유통계학의의(균P＜0.05).농독증대서12 h시유핵세포PPARγ활성여혈장IL-6수평정현저부상관(r=-0.703,P=0.023).결론 농독증유핵세포PPARγ활성하강,혈장촉염개질IL-6승고,차량자정부상관.
Objective To observe the relationship between activity of peroxisome proliferator activated receptor γ (PPARγ) in nucleated cell and level of pro-inflammatory mediator interleukin-6 (IL-6) in plasma of rats with sepsis. Methods According to the random number table, 90 male Sprague-Dawley (SD)rats were randomly divided into three groups, namely control group, sham operation group and sepsis group.Each group was further divided into three subgroups according to postoperative time points, i.e. 12, 24 and 48-hour subgroups. Each subgroup consisted of 10 rats. Sepsis was reproduced by cecal ligation and puncture (CLP). The PPARγ activity in nucleated cells and IL-6 level in plasma were detected by enzyme-linked immunosorbent assay (ELISA). Results The PPARγ activity in nucleated cells was significantly decreased at 12, 24 and 48 hours in sepsis group (A value: 0. 279±0. 004, 0. 264±0. 009, 0. 245±0. 012) compared with control group (0. 292 ± 0. 007, 0.293 ±0.004, 0.293 ±0.005) and sham operation group (0. 295 ±0.008, 0.295 ±0.006, 0. 294 ± 0. 007), while the IL-6 level was significantly increased in sepsis group (ng/L; 365. 25 ±15. 53, 507.16 ±20. 86, 437. 89 ±25.09) compared with control group (43. 54 ± 11.10,48. 82±10. 62, 42. 96±9. 52) and sham operation group (42. 43±6. 77, 40. 32±6. 48, 44.10±9. 36, all P＜0. 05). When septic condition became worse, the PPARγ activity in nucleated cells of sepsis group lowered,and IL-6 level was gradually elevated after operation, reaching the peak at 24 hours, and then gradually lowered, and the difference of the value between any two time points was all statistically significant (all P＜0. 05). There was a negative correlation between the PPARγ activity in nucleated cells and IL-6 level in 12-hour subgroup of sepsis group (r=-0. 703, P=0. 023). Conclusion In septic rats, the PPARγ activity in nucleated cells was lowered while the pro-inflammatory mediator IL-6 level in plasma elevated, and there was a negative correlation between PPARγ activity and IL-6 level.