CAJ | 학술논문

目的探讨肝细胞肝癌中肝肠钙黏连蛋白( LI-cadherin)的mRNA和蛋白表达水平及其临床意义。方法应用逆转录-聚合酶链反应( RT-PCR)及免疫组织化学方法检测LI-cadherin的mRNA及蛋白在56例肝癌组织、44例癌旁肝组织和9例正常肝组织中的表达水平，并分析其与临床病理学特征之间的关系。结果肝癌组织和癌旁肝组织中均有Ll-cadherin mRNA的表达，正常肝组织中均未枪测到LI-cadherin mRNA的表达。LI-cadherin mRNA在肝癌组织中的表达显著高于在癌旁肝组织中的表达(0.653±0.147 vs 0.534±0.138)，且两者之间的差异有统计学意义(P＜0.01)。LI-cadherin蛋白在肝癌组织中其阳性表达率为64.29％( 36/56)，在癌旁组织中其阳性表达率为22.73％(10/44)，在正常肝组织中没有发现其表达。LI-cadherin蛋白的过表达与肝癌细胞的分化程度、淋巴结转移及门静脉癌栓的形成等临床病理因素有关(P＜0.05)。结论 LI-cadherin在肝癌细胞的发生、发展过程中发挥重要作用。LI-cadherin蛋白的过表达与肝癌细胞的淋巴结转移和门静脉癌栓有关，提示其在肝癌的侵袭和转移中发挥重要的作用。
목적 탐토간세포간암중간장개점련단백( LI-cadherin)적mRNA화단백표체수평급기림상의의。방법 응용역전록-취합매련반응( RT-PCR)급면역조직화학방법검측LI-cadherin적mRNA급단백재56례간암조직、44례암방간조직화9례정상간조직중적표체수평，병분석기여림상병이학특정지간적관계。결과 간암조직화암방간조직중균유Ll-cadherin mRNA적표체，정상간조직중균미창측도LI-cadherin mRNA적표체。LI-cadherin mRNA재간암조직중적표체현저고우재암방간조직중적표체(0.653±0.147 vs 0.534±0.138)，차량자지간적차이유통계학의의(P＜0.01)。LI-cadherin단백재간암조직중기양성표체솔위64.29％( 36/56)，재암방조직중기양성표체솔위22.73％(10/44)，재정상간조직중몰유발현기표체。LI-cadherin단백적과표체여간암세포적분화정도、림파결전이급문정맥암전적형성등림상병리인소유관(P＜0.05)。결론 LI-cadherin재간암세포적발생、발전과정중발휘중요작용。LI-cadherin단백적과표체여간암세포적림파결전이화문정맥암전유관，제시기재간암적침습화전이중발휘중요적작용。
ObjectiveTo explore the expression and clinical significance of LI ( liver-intestine)-cadherin mRNA and protein expression level in hepatocellular carcinoma. MethodsReverse transcriptionpolymerase chain reaction(RT-PCR)and immunohistochemical techniques were used to detect the expression of the LI-cadherin mRNA and protein in 56 cases of hepatocellular carcinoma, 44 cases of corresponding paracancerous tissues and 9 cases of normal liver tissues.The relationships with its clinicopathological characteristics were analyzed. ResultsThe mRNA of LI-cadherin was expressed in 56 cases of hepatocellular carcinoma tissues and 44 cases of corresponding paracancerous tissues.But its expression was not detected in normal tissues. Semiquantitative analysis showed that the mRNA expression level of LI-caherin was greater in hepatocellular carcinoma tissues than that in corresponding paracancerous tissues (0. 653 ± 0. 147 vs 0. 534 ± 0. 138 ). The differences were statistically significant (P ＜ 0. 01 ). The positive rates of LI-cadherin protein were 64. 29％ (36/56) in hepatocellular carcinoma tissues, 22.73％(10/44) in paracancerous tissues and 0％ (0/9) in normal tissues. ConclusionThe expression of LI-cadherin is up-regulated significantly in HCC. The over-expression of LI-cadherin protein is correlated with the lymph node metastasis and tumor thrombi in portal vein. It indicates that LI-cadherin may play an important role in the progression and metastasis of HCC.