CAJ | 학술논문

目的探讨小干扰RNA(siRNA)调节导致的△Np73抑制对结肠癌细胞SW620 5-氟尿嘧啶(5-FU)药物敏感性的影响,为结肠癌治疗提供新途径.方法将△Np73 siRNA转染人SW620结肠癌细胞,观察其对结肠癌细胞△Np73表达的影响.并与5-FU联合使用,四甲基偶氮唑盐比色(MTT)法检测细胞活力,流式细胞技术检测细胞凋亡.分别将转染△Np73 siRNA和阴性对照siRNA的SW620细胞注射裸鼠成瘤,瘤中注射5-FU观察体内肿瘤的生长情况.结果 △Np73 siRNA可显著抑制SW620结肠癌细胞△Np73的表达,但本身均不能抑制SW620结肠癌细胞的生长.同时应用△Np73 siRNA和5-FU共同处理的SW620细胞的凋亡率达到42.9%,显著高于单纯5-FU处理组(18.9%)和单纯△Np73处理组(8.8%).在转染△Np73 siRNA的成瘤小鼠的瘤中注射5-FU,能明显抑制癌细胞体外生长(t=15.32,P<0.05).结论 △Np73 siRNA可通过抑制△Np73的表达,从而增强对化疗药物的敏感性.
목적 탐토소간우RNA(siRNA)조절도치적△Np73억제대결장암세포SW620 5-불뇨밀정(5-FU)약물민감성적영향,위결장암치료제공신도경.방법 장△Np73 siRNA전염인SW620결장암세포,관찰기대결장암세포△Np73표체적영향.병여5-FU연합사용,사갑기우담서염비색(MTT)법검측세포활력,류식세포기술검측세포조망.분별장전염△Np73 siRNA화음성대조siRNA적SW620세포주사라서성류,류중주사5-FU관찰체내종류적생장정황.결과 △Np73 siRNA가현저억제SW620결장암세포△Np73적표체,단본신균불능억제SW620결장암세포적생장.동시응용△Np73 siRNA화5-FU공동처리적SW620세포적조망솔체도42.9%,현저고우단순5-FU처리조(18.9%)화단순△Np73처리조(8.8%).재전염△Np73 siRNA적성류소서적류중주사5-FU,능명현억제암세포체외생장(t=15.32,P<0.05).결론 △Np73 siRNA가통과억제△Np73적표체,종이증강대화료약물적민감성.
Objective To investigate the effect of small interfering RNA (siRNA) mediated silencing of △Np73 on 5-FU chemotherapy sensitivity in SW620 colocancer cells and provide new treatment approach for the colon cancer.Methods siRNAs were transfected into SW620 colon cancer cells.The expression of △Np73 was observed.Cell viability of colon cancer cells were measured by MTr assay and cell apoptosis was assessed with flow cytometry after treatment of control siRNA or △Np73 siRNA or combined with 5-FU,respectively.The tumorigenesis was assessed by injecting △Np73 siRNA or control siRNA transfeeted SW620 colon cancer cells into nude mice,followed by treatment with 5-FU in the tumors.Results △Np73 siRNA was able to strongly inhibit △Np73 expression,however,it did not inhibit the growth of cells.Combination treatment with △Np73 siRNA and 5-FU produced significant higher apoptotic cell(42.9%) as compared with those with 5-FU treatment(18.9%) alone or those with △Np73 siRNA(8.8%) alone.The treatment with 5-FU in the xenngrafts derived from △Np73 siRNA transfected SW620 cells in nude mice can inhibitor tumor growth significantly (t=15.32,P<0.05).Conclusion △Np73siRNAs can specifically repress the expression of △Np73.Thus it sensitizess the cells to 5-FU chemotherapy in colon cancer.