CAJ | 학술논문

目的探讨磷脂酰肌醇3-激酶(PI3-K)基因P85α调节亚单位Met326Ile突变及基因表达与2型糖尿病(T2DM)的关系. 方法采用双引物竞争PCR法检测T2DM者240例及正常对照(NC)组150例Met326Ile突变,对部分受检者的外周血白细胞RNA进行RT-PCR扩增,观察PI3-K基因表达水平. 结果 T2DM患者与NC组相比,P85α基因Ile326等位基因频率分别为22.5%和22.4%(P＞0.05),三种基因型间BMI、FIns、HOMA-IR及基因表达水平差异均无统计学意义(P＞0.01),T2DM患者的基因表达水平明显低于NC组(P＜0.01). 结论 PI3-K P85α基因Met326Ile错义突变可能为一无功能性突变,其与T2DM及PI3-K基因表达无相关.
목적 탐토린지선기순3-격매(PI3-K)기인P85α조절아단위Met326Ile돌변급기인표체여2형당뇨병(T2DM)적관계. 방법 채용쌍인물경쟁PCR법검측T2DM자240례급정상대조(NC)조150례Met326Ile돌변,대부분수검자적외주혈백세포RNA진행RT-PCR확증,관찰PI3-K기인표체수평. 결과 T2DM환자여NC조상비,P85α기인Ile326등위기인빈솔분별위22.5%화22.4%(P＞0.05),삼충기인형간BMI、FIns、HOMA-IR급기인표체수평차이균무통계학의의(P＞0.01),T2DM환자적기인표체수평명현저우NC조(P＜0.01). 결론 PI3-K P85α기인Met326Ile착의돌변가능위일무공능성돌변,기여T2DM급PI3-K기인표체무상관.
Objective To investigate the impact of Met326Ile variant of the p85α subunit of PI3-K and its gene expression on type 2 diabetes.Methods The Met326Ile mutation was determined by polymerase chain reaction(PCR) in 240 diabetes inpatients and 150 healthy controls.RT-PCR and spot density measurement were employed to estimate gene expression. Results The allelic frequency of the Ile326 variant was similar in type 2 diabetes and control(22.5% vs 22.4%,P>0.05),and the distribution of genotypes was in Hardy-Weinberg equilibrium.Data analysis showed Met326Ile variant did not impact on FIns,FPG,HOMA index,BMI and gene expression of PI3-K,though the expression of PI3-K gene was lower in type 2 diabetes than in control group.Conclusion The Met326Ile variant of the p85α regulatory subunit of PI3-K is likely to be functionally normal in type 2 diabetes and health people.