CAJ | 학술논문

目的检测直肠癌及癌旁组织中DNA含量以探讨直肠癌远端肠管的切除范围.方法采用流式细胞分析技术对60例直肠癌标本的肿瘤组织(T)、远端1 cm(P1)、远端2 cm(P2)、远端3 cm(P3)、远端4 cm(P4)、远端5cm(P5)及正常组织(N)进行检测,分析各部位组织中DNA指数(DI)的差异性.结果 T组织异倍体率显著高于P1～P5组织及N组织,P4组织异倍体率显著高于P5及N组织,而P5与N组织筹异无显著性.结论直肠癌远端1～4 cm肠管中存在着DNA含量的异常改变,呈现出恶性肿瘤生物学特性.手术时应切除癌旁超过4 cm作为安全切除范围.
목적 검측직장암급암방조직중DNA함량이탐토직장암원단장관적절제범위.방법 채용류식세포분석기술대60례직장암표본적종류조직(T)、원단1 cm(P1)、원단2 cm(P2)、원단3 cm(P3)、원단4 cm(P4)、원단5cm(P5)급정상조직(N)진행검측,분석각부위조직중DNA지수(DI)적차이성.결과 T조직이배체솔현저고우P1～P5조직급N조직,P4조직이배체솔현저고우P5급N조직,이P5여N조직주이무현저성.결론 직장암원단1～4 cm장관중존재착DNA함량적이상개변,정현출악성종류생물학특성.수술시응절제암방초과4 cm작위안전절제범위.
Objective To explore the optimal distence of distal resection margin of rectal carcinoma by detecting DNA contents.Methods Sixty cases of colorectal carcinoma(T),tissue 1 cm(P1),2 cm (P2),3 cm(P3),4 cm(P4),5 cm(P5)away from tumor margin and the normal tissue(N)were detected by the flow cytometry.DNA index(DI)were analyzed.Results The aneuploidy rate in tumors were significantly higher than that in PI～P5 and normal tissues,meanwhile the rate in P4 significantly higher than that in P5 and normal tissues.While no significant difference of aneuploidy rate was found between P5 and normal tissues.Conclusion The abnormal change of DNA contents can be detected in the 4 cm distal rectum,it shows the tendency of malignancy.Beyond 4 am tissue of tumor margin should be removed in curative procedure for rectal carcinoma.