CAJ | 학술논문

采用阳离子交换色谱-直接电导法测定部分常见氨基酸和无机阳离子,以酒石酸和吡啶二羧酸溶液为淋洗液,利用阳离子色谱系统等度洗脱,通过对色谱分析条件的优化,无需衍生化即可同时测定谷氨酸、丙氨酸、甲硫氨酸、亮氨酸、苯丙氨酸、赖氨酸及Na~+,NH_4~+,K~+,Ca~(2+)和Mg~(2+).选择12 min内出峰的5种氨基酸和3种无机阳离子进行定量分析,结果表明,其相对标准偏差RSD_(n=5)≤4.690%,标准曲线的线性相关系数不低于0.9984,检出限在0.27～10.34 mg/L之间.以建立的方法测定味精等电母液中的氨基酸,结果显示,氨基酸的回收率为88.7%～107.2%.
채용양리자교환색보-직접전도법측정부분상견안기산화무궤양리자,이주석산화필정이최산용액위림세액,이용양리자색보계통등도세탈,통과대색보분석조건적우화,무수연생화즉가동시측정곡안산、병안산、갑류안산、량안산、분병안산、뢰안산급Na~+,NH_4~+,K~+,Ca~(2+)화Mg~(2+).선택12 min내출봉적5충안기산화3충무궤양리자진행정량분석,결과표명,기상대표준편차RSD_(n=5)≤4.690%,표준곡선적선성상관계수불저우0.9984,검출한재0.27～10.34 mg/L지간.이건립적방법측정미정등전모액중적안기산,결과현시,안기산적회수솔위88.7%～107.2%.
A simple, rapid and accurate method for simultaneous determination of six amino acids (glutamic acid, alanine, methionine, leucine, phenylalanine and lysine) and five inorganic cations (Na~+, NH_4~+, K~+, Ca~(2+) and Mg~(2+)) was developed. The quantitative determination of these ions was accomplished by ion chromatography with direct conductivity detection based on their ionization in acidic medium without chemical suppression and derivatization. In a single chromatographic run, the five amino acids (glutamic acid, alanine, methionine, leucine and phenylalanine) and three inorganic cations (Na~+, NH_4~+ and K~+) were separated and determined within 12 min. The relative standard deviation (RSD, n=5) was less than 4.690%. The detection limits calculated were in the range from 0.27 to 10.34 mg/L. All the correlation coefficients (R~2) of these ions were not less than 0.9984. The recovery rates of some amino acids in isoelectric supernant of monosodium glutamate were in the range from 88.7% to 107.2%.