CAJ | 학술논문

从NCBI 数据库(http:∥www.ncbi.nlm.nih.gov/projects/mapview/map)下载鸡Z染色体上全部基因完整的cDNA,最终共有626个基因的CDS序列纳入统计分析.使用CodonW(1.4.2)进行密码子偏性分析,确定了CGG、AGC、UGC等26个密码子为Z染色体基因表达的"最优"密码子.对应分析表明,影响鸡Z染色体基因表达的密码子偏性的主要因素分别为GC3s、基因的表达丰度、GC含量、CDS长度及氨基酸的疏水性.鸡Z染色体基因表达的密码子用法受到了核苷酸组成偏好的显著影响,这种核苷酸组成偏好很可能是突变偏畸、固定偏畸及基因转换导致的.对于鸡这种群体有效规模较大的群体,密码子的偏性更有可能是核苷酸组成偏好及选择等因素综合作用的结果.
종NCBI 수거고(http:∥www.ncbi.nlm.nih.gov/projects/mapview/map)하재계Z염색체상전부기인완정적cDNA,최종공유626개기인적CDS서렬납입통계분석.사용CodonW(1.4.2)진행밀마자편성분석,학정료CGG、AGC、UGC등26개밀마자위Z염색체기인표체적"최우"밀마자.대응분석표명,영향계Z염색체기인표체적밀마자편성적주요인소분별위GC3s、기인적표체봉도、GC함량、CDS장도급안기산적소수성.계Z염색체기인표체적밀마자용법수도료핵감산조성편호적현저영향,저충핵감산조성편호흔가능시돌변편기、고정편기급기인전환도치적.대우계저충군체유효규모교대적군체,밀마자적편성경유가능시핵감산조성편호급선택등인소종합작용적결과.
The total cDNA sequences were downloaded from NCBI dateset,and 626 CDS sequences were finally included in analyses.By the use of CodonW(1.4.2),26 codons (eg.CGG,AGC,UGC et al.) were identified as optimal codons.The corresponding analyses indicted that the main factors influencing codon usage of genes in GGAZ were GC3s,expression level,GC content,CDS length as well as Hydrophobicity of amino acids.As the codon usage of genes in GGAZ was mostly influcenced by nucleotide composition,the bias of nucleotide composition is more likely due to the mutation bias,fix bias,and gene conversion.For a greater effective population size,the codon usage of chicken was most likely the combination of nucleotide composition and selection and so on.