CAJ | 학술논문

为初步探讨染锰对公鸡肝细胞凋亡的影响,将400只50日龄海兰褐公鸡随机分为4组,分别在饲料中添加0、600、900、1 800 mg·kg~(-1) MnCl_2建立亚慢性锰中毒模型,于染毒第30、60、90天采取肝脏观察肝细胞形态学变化,检测肝细胞凋亡指数以及分析Fas mRNA的转录水平.结果显示,在第90天时,高剂量组与对照组相比,锰中毒诱导出现典型凋亡形态变化,随着染毒剂量的升高,凋亡细胞的数量逐渐增多;不同时间点,随着染锰剂量的增加,Fas基因mRNA转录水平基本呈升高趋势.
위초보탐토염맹대공계간세포조망적영향,장400지50일령해란갈공계수궤분위4조,분별재사료중첨가0、600、900、1 800 mg·kg~(-1) MnCl_2건립아만성맹중독모형,우염독제30、60、90천채취간장관찰간세포형태학변화,검측간세포조망지수이급분석Fas mRNA적전록수평.결과현시,재제90천시,고제량조여대조조상비,맹중독유도출현전형조망형태변화,수착염독제량적승고,조망세포적수량축점증다;불동시간점,수착염맹제량적증가,Fas기인mRNA전록수평기본정승고추세.
The aim of this study was to investigate the effect of manganese on the hepatic apoptosis in cocks. 400 fifty-day old Hy-line brown cocks were randomly divided into four groups. The 0, 600, 900 and 1 800 mg·kg~(-1) MnCl_2 were respectively added into the basic forage to establish the subchronic manganism model. After 30, 60 and 90-day treatment, the livers in every group were collected to observe hepatic morphological change and detect hepatic apoptosis index and the transcription level of Fas mRNA. Results showed that among 90-day treatment groups, the liver form the highest dose group took on the typical morphologic changes of apoptosis compared with the corresponding control group, and the number of the apoptotic cells gradually increased with the increase of the manganese dose. The transcription level of Fas mRNA also took on the doseeffect relationship at different treatment time.