CAJ | 학술논문

目的:观察EPA、DHA对脂多糖(LPS)刺激大鼠系膜细胞(GMCs)的氧化应激及MCP-1和TGF-β_1表达的影响.方法:用LPS(10mg/L)刺激大鼠GMCs,经EPA(10 μmol/L、100 μmol/L)、DHA(10 μmol/L、100 μmol/L)培养,采用试剂盒分别测定培养24 h、48 h、72 h培养上清中的SOD、GSH-Px和MDA.采用免疫细胞化学方法测定培养系膜细胞MCP-1和TGF-β_1的表达,采用实时定量PCR方法测定MCP-1和TGF-β_1 mRNA的表达.结果:LPS刺激后,系膜细胞SOD、GSH-Px活力降低,MDA含量增加.EPA、DHA能明显升高SOD、GSH-Px活力,减少MDA生成.LPS刺激可使系膜细胞表达MCP-1、TGF-β_1增加,经EPA、DHA处理后,MCP-1、TGF-β_1 mRNA和蛋白表达明显减少.DHA作用强于同剂量EPA.结论:EPA、DHA能提高系膜细胞的抗氧化酶SOD、GSH-Px活力,减少细胞内MDA生成.EPA和DHA可以降低LPS刺激的GMCs的TGF-β_1与MCP-1的表达,从而起到保护肾功能的作用.
목적:관찰EPA、DHA대지다당(LPS)자격대서계막세포(GMCs)적양화응격급MCP-1화TGF-β_1표체적영향.방법:용LPS(10mg/L)자격대서GMCs,경EPA(10 μmol/L、100 μmol/L)、DHA(10 μmol/L、100 μmol/L)배양,채용시제합분별측정배양24 h、48 h、72 h배양상청중적SOD、GSH-Px화MDA.채용면역세포화학방법측정배양계막세포MCP-1화TGF-β_1적표체,채용실시정량PCR방법측정MCP-1화TGF-β_1 mRNA적표체.결과:LPS자격후,계막세포SOD、GSH-Px활력강저,MDA함량증가.EPA、DHA능명현승고SOD、GSH-Px활력,감소MDA생성.LPS자격가사계막세포표체MCP-1、TGF-β_1증가,경EPA、DHA처리후,MCP-1、TGF-β_1 mRNA화단백표체명현감소.DHA작용강우동제량EPA.결론:EPA、DHA능제고계막세포적항양화매SOD、GSH-Px활력,감소세포내MDA생성.EPA화DHA가이강저LPS자격적GMCs적TGF-β_1여MCP-1적표체,종이기도보호신공능적작용.
AIM: To investigate the effects of EPA and DHA on oxidative stress of lipopolysaccharide-stimulated rat mesangial cells. METHODS: The glomerular mesangial cells (GMCs) were stimulated by lipopolysaccharide (LPS) and incubated with EPA (10 μmol/L or 100 μmol/L) and DHA (10 μmol/L or 100 μmol/L) for 24 h, 48 h and 72 h. The activity of SOD, GSH-Px and the level of MDA was measured. The protein and mRNA expressions of MCP-1 and TGF-β_1 were detected by immunocytochemistry and real-time PCR method, respectively. RESULTS: The activities of SOD and GSH-Px were decreased and the concentration of MDA was increased when stimulated with LPS. EPA and DHA increased the activities of SOD and GSH-Px and decreased the concentration of MDA significantly. Meanwhile, the protein and mRNA expressions of MCP-1 and TGF-β_1 stimulated by LPS were decreased. DHA was more effective than EPA at the same concentration. CONCLUSION: EPA and DHA enhance the activities of antioxidant enzymes, decrease the concentration of MDA and inhibit the expression of TGF-β_1 and MCP-1, suggesting that the protective effect of EPA and DHA on kidney is related to the antioxidation and the inhibition of TGF-β_1 and MCP-1 expression.