CAJ | 학술논문

目的研究A型与B型CpG ODN对人外周血单个核细胞(PBMC)的增殖,细胞因子的释放和诱导人PBMC抗膀胱肿瘤细胞作用.方法用A型、B型CpG ODN和NoCPG ODN刺激正常人外周血PBMC,MTT测定细胞的增殖,定量ELISA检测人干扰素-α含量,LDH法检测CpG ODN诱导后人PBMC对膀胱肿瘤细胞的杀伤作用.结果 A型与B型CpG ODN对人PBMC增殖的刺激作用明显高于对照组；CpGODN2216组IFN-α的浓度高于CPG ODN2006组；CpG寡核苷酸作用后,人PBMC对BIU-87膀胱肿瘤细胞的杀伤活性明显升高.结论 A型与B型CpG ODN对人PBMC具有免疫诱导作用,可刺激人PBMC增殖并释放IFN-α,诱导免疫细胞对膀胱肿瘤细胞杀伤,增强宿主抗膀胱肿瘤细胞作用.
목적 연구A형여B형CpG ODN대인외주혈단개핵세포(PBMC)적증식,세포인자적석방화유도인PBMC항방광종류세포작용.방법 용A형、B형CpG ODN화NoCPG ODN자격정상인외주혈PBMC,MTT측정세포적증식,정량ELISA검측인간우소-α함량,LDH법검측CpG ODN유도후인PBMC대방광종류세포적살상작용.결과 A형여B형CpG ODN대인PBMC증식적자격작용명현고우대조조；CpGODN2216조IFN-α적농도고우CPG ODN2006조；CpG과핵감산작용후,인PBMC대BIU-87방광종류세포적살상활성명현승고.결론 A형여B형CpG ODN대인PBMC구유면역유도작용,가자격인PBMC증식병석방IFN-α,유도면역세포대방광종류세포살상,증강숙주항방광종류세포작용.
Objectives The study was designed to investigate the function of the effect of Type A and B CpG oligodeoxynucleotides on the differentiation,cytokine secreted by PBMC,cytotype change of B cells.Methods To test lymphocyte proliferation by MTT method after human PBMC was incubated with type A and B CpG ODN;To measure cytokine IFN - α release by ELISA on human PBMC induced by CpG ODN;Analysis B cell surface marker of human PBMC by flow cytometry.Results Both type A and B CpG ODN could induce proliferation of PBMC.Induct effection of type A is significantly higher than type B.Type A CpG ODN strongly induce PDCs to secrete IFN -α.Type B CpG ODNs induce less IFN -α and primarily activate B cells,causing them to up -regulate CD69.Conclusions CpG ODN could have immune induction of human PBMC and up - regulate immune function.