中华普通外科杂志
中華普通外科雜誌
중화보통외과잡지
CHINESE JOURNAL OF GENERAL SURGERY
2010年
5期
401-404
,共4页
陈曦%韦瑶%张强%苏畅%张正筠%张明均%周光文
陳晞%韋瑤%張彊%囌暢%張正筠%張明均%週光文
진희%위요%장강%소창%장정균%장명균%주광문
胰岛移植%模型,动物%肝星状细胞
胰島移植%模型,動物%肝星狀細胞
이도이식%모형,동물%간성상세포
Islet of langerhans transplantation%Models,animal%Hepatic stellate cells
目的 研究小鼠肝星状细胞对同种异体胰岛移植物的保护作用.方法 将糖尿病小鼠随机分为三组,分别为糖尿病组、单纯胰岛移植组及与肝星状细胞共同移植组.单纯移植组于肾被膜下移植入同种异体胰岛300个;共同移植组移植入肝星状细胞(3×105个)与同种异体胰岛(300个)混合物.分别于移植术后监测受体小鼠血糖值及正常血糖维持时间;血糖正常一周后移植组及糖尿病组小鼠分别采血检测血清中TGF-β,TNF-α,IL-1β,IFN-γ的含量,同时取出移植物进行免疫组织化学检测.结果 术后共同移植组受体正常血糖维持时间为(23.75±8.96)d,单纯胰岛移植组受体正常血糖维持时间为(11.9±6.92)d,差异有统计学意义(P<0.05);三组受体血清中TNF-α、IL-1β、IFN-γ含量差异无统计学意义(P>0.05),共同移植组受体血清中TGF-β含量为(2292.31±5.87)pg/ml,单纯移植组为(1246.55±38.91)pg/ml,两组比较差异有统计学意义(P<0.05);病理学结果显示共同移植组胰岛素表达量大,并且在移植物周围有生物包膜形成.结论 在同种异体移植模型中,肝星状细胞可能通过高分泌TGF-β、局部形成包囊等方式保护胰岛移植物并延长其存活时间.
目的 研究小鼠肝星狀細胞對同種異體胰島移植物的保護作用.方法 將糖尿病小鼠隨機分為三組,分彆為糖尿病組、單純胰島移植組及與肝星狀細胞共同移植組.單純移植組于腎被膜下移植入同種異體胰島300箇;共同移植組移植入肝星狀細胞(3×105箇)與同種異體胰島(300箇)混閤物.分彆于移植術後鑑測受體小鼠血糖值及正常血糖維持時間;血糖正常一週後移植組及糖尿病組小鼠分彆採血檢測血清中TGF-β,TNF-α,IL-1β,IFN-γ的含量,同時取齣移植物進行免疫組織化學檢測.結果 術後共同移植組受體正常血糖維持時間為(23.75±8.96)d,單純胰島移植組受體正常血糖維持時間為(11.9±6.92)d,差異有統計學意義(P<0.05);三組受體血清中TNF-α、IL-1β、IFN-γ含量差異無統計學意義(P>0.05),共同移植組受體血清中TGF-β含量為(2292.31±5.87)pg/ml,單純移植組為(1246.55±38.91)pg/ml,兩組比較差異有統計學意義(P<0.05);病理學結果顯示共同移植組胰島素錶達量大,併且在移植物週圍有生物包膜形成.結論 在同種異體移植模型中,肝星狀細胞可能通過高分泌TGF-β、跼部形成包囊等方式保護胰島移植物併延長其存活時間.
목적 연구소서간성상세포대동충이체이도이식물적보호작용.방법 장당뇨병소서수궤분위삼조,분별위당뇨병조、단순이도이식조급여간성상세포공동이식조.단순이식조우신피막하이식입동충이체이도300개;공동이식조이식입간성상세포(3×105개)여동충이체이도(300개)혼합물.분별우이식술후감측수체소서혈당치급정상혈당유지시간;혈당정상일주후이식조급당뇨병조소서분별채혈검측혈청중TGF-β,TNF-α,IL-1β,IFN-γ적함량,동시취출이식물진행면역조직화학검측.결과 술후공동이식조수체정상혈당유지시간위(23.75±8.96)d,단순이도이식조수체정상혈당유지시간위(11.9±6.92)d,차이유통계학의의(P<0.05);삼조수체혈청중TNF-α、IL-1β、IFN-γ함량차이무통계학의의(P>0.05),공동이식조수체혈청중TGF-β함량위(2292.31±5.87)pg/ml,단순이식조위(1246.55±38.91)pg/ml,량조비교차이유통계학의의(P<0.05);병이학결과현시공동이식조이도소표체량대,병차재이식물주위유생물포막형성.결론 재동충이체이식모형중,간성상세포가능통과고분비TGF-β、국부형성포낭등방식보호이도이식물병연장기존활시간.
Objective To analyze the protection supplied by HSCs in vivo after islet cells homotransplantation. Method Diabetic mice were randomly divided into three groups as diabetic group,islet-transplant group and co-transplant group. 300 islets alone or mixed with HSCs were transplanted under the capsule of the kidney of the diabetic recipients respectively. Blood glucose and the length of normal blood glucose level were recorded and we collected the blood and tissue of islet graft 7 days after transplantation in each group. Blood concentration of TGF-β, TNF-α, IL-1β and IFN-γ were detected by ELISA. The infiltration of lymphocytes was observed by HE staining and immunohistochemieal examination.Result Co-transplant group had a prolonged islet allograft survival for (23.75 ± 8. 96) days, compared with the islet alone of (11.9 ± 6. 92) days. Blood concentration of TGF-β in co-transplant group was (2292.31 ± 5.87) pg/ml, significantly higher than those in simple transplant group (1246.55 ±38.91) pg/ml(P <0.05), there was no differentence in the two groups for TNF-α,IL-1β and IFN-γ.Conclusion HSCs may prolong the islet graft survival by expressing higher level of TGF-β and form a capsule around the graft.
