中华航海医学与高气压医学杂志
中華航海醫學與高氣壓醫學雜誌
중화항해의학여고기압의학잡지
CHINESE JOURNAL OF NAUTICAL MEDICINE AND HYPERBARIC MEDICINE
2011年
6期
347-350
,共4页
祝子鹏%秦军%孙俊波%汤敏%吴建平%闫玉华%詹金凤%李厚成
祝子鵬%秦軍%孫俊波%湯敏%吳建平%閆玉華%詹金鳳%李厚成
축자붕%진군%손준파%탕민%오건평%염옥화%첨금봉%리후성
高压氧%亚低温%细胞凋亡%创伤性脑损伤%Bcl -2
高壓氧%亞低溫%細胞凋亡%創傷性腦損傷%Bcl -2
고압양%아저온%세포조망%창상성뇌손상%Bcl -2
Hyperbaric oxygenation%Mild hypothermia%Apoptosis%Traumatic brain injury%Bcl-2
目的 探讨高压氧(HBO)联合亚低温(MHT)治疗对创伤性脑损伤(TBI)细胞凋亡及Bcl-2表达的影响.方法 制作大鼠自由落体脑挫伤动物模型,设立TBI常规治疗组(TBI组)、常规治疗+ HBO组、常规治疗+MHT组和常规治疗+HBO+ MHT组,应用原位末端标记法(TUNEL)和流式细胞术对各组大鼠脑挫伤病灶旁的细胞凋亡情况进行检测;使用免疫组化方法检测Bcl-2蛋白在脑组织中的表达情况.结果 常规治疗+ HBO组(16.3±5.0)、常规治疗+MHT组(18.7±6.9)和常规治疗+HBO+ MHT组(24.7±7.2) Bcl-2的表达均高于常规治疗组(8.9±4.2)(P<0.05),其中常规治疗+HBO+MHT组表达最高.结论 HBO联合亚低温治疗脑创伤可有效减少细胞凋亡及增加Bcl-2的表达.
目的 探討高壓氧(HBO)聯閤亞低溫(MHT)治療對創傷性腦損傷(TBI)細胞凋亡及Bcl-2錶達的影響.方法 製作大鼠自由落體腦挫傷動物模型,設立TBI常規治療組(TBI組)、常規治療+ HBO組、常規治療+MHT組和常規治療+HBO+ MHT組,應用原位末耑標記法(TUNEL)和流式細胞術對各組大鼠腦挫傷病竈徬的細胞凋亡情況進行檢測;使用免疫組化方法檢測Bcl-2蛋白在腦組織中的錶達情況.結果 常規治療+ HBO組(16.3±5.0)、常規治療+MHT組(18.7±6.9)和常規治療+HBO+ MHT組(24.7±7.2) Bcl-2的錶達均高于常規治療組(8.9±4.2)(P<0.05),其中常規治療+HBO+MHT組錶達最高.結論 HBO聯閤亞低溫治療腦創傷可有效減少細胞凋亡及增加Bcl-2的錶達.
목적 탐토고압양(HBO)연합아저온(MHT)치료대창상성뇌손상(TBI)세포조망급Bcl-2표체적영향.방법 제작대서자유락체뇌좌상동물모형,설립TBI상규치료조(TBI조)、상규치료+ HBO조、상규치료+MHT조화상규치료+HBO+ MHT조,응용원위말단표기법(TUNEL)화류식세포술대각조대서뇌좌상병조방적세포조망정황진행검측;사용면역조화방법검측Bcl-2단백재뇌조직중적표체정황.결과 상규치료+ HBO조(16.3±5.0)、상규치료+MHT조(18.7±6.9)화상규치료+HBO+ MHT조(24.7±7.2) Bcl-2적표체균고우상규치료조(8.9±4.2)(P<0.05),기중상규치료+HBO+MHT조표체최고.결론 HBO연합아저온치료뇌창상가유효감소세포조망급증가Bcl-2적표체.
Objective To investigate the effects of hyperbaric oxygen (HBO) coupled with mild hypothermia (MHT) on apoptosis and expression of Bcl-2 following traumatic brain injury (TBI).Methods The rat model of traumatic brain injury was developed with the free fall method,then,the animals were randomly assigned to one of the four groups: the routine therapy group,the routine therapy + HBO group,the routine therapy + MHT group,and the routine therapy + HBO + MHT group.Apoptosis of neuron cells adjacent to the traumatic cerebral injury foci was evaluated with TUNEL and flow cytometry,and the expression of Bcl-2 in the brain tissue was detected with immunohistochemistry.Results The expressions,of Bcl-2 in the routine therapy + HBO( 16.3 ±5.0),the routine therapy + MHT( 18.7 ±6.9),and the routine therapy + HBO + MHT groups( 24.7 ± 7.2 ) were all higher than that of the pure routine therapy group ( 8.9 ± 4.2) ( P < 0.05 ),with the expression of Bcl-2 in the routine therapy + HBO + MHT group being the highest.Conclusions HBO coupled with MHT could suppress the apoptosis and increase the expression of Bcl-2 efficiently.