中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2011年
23期
1613-1616
,共4页
阮菲%吴瑞瑾%徐开红%马俊彦%鲁东红%陈利青%涂飞霞%梁峥%林俊
阮菲%吳瑞瑾%徐開紅%馬俊彥%魯東紅%陳利青%塗飛霞%樑崢%林俊
원비%오서근%서개홍%마준언%로동홍%진리청%도비하%량쟁%림준
子宫内膜异位症%肝再生磷酸酶-3%小干扰RNA%迁移
子宮內膜異位癥%肝再生燐痠酶-3%小榦擾RNA%遷移
자궁내막이위증%간재생린산매-3%소간우RNA%천이
Endometriosis%Phosphatase of regenerating liver-3%Small interfering RNA%Migration
目的 通过siRNA体外沉默肝再生磷酸酶-3(PRL-3),探讨其对异位子宫内膜间质细胞迁移的影响.方法 原代培养异位子宫内膜间质细胞,运用siRNA技术干扰沉默PRL-3基因,Western blot检测干扰后PRL-3蛋白表达变化,观察细胞板状伪足的形成,采用划痕实验、transwell细胞迁移实验观察并比较细胞迁移能力的改变.结果 经siRNA 干扰沉默后实验组PRL-3蛋白相对表达量较对照组明显下降(P<0.05),细胞形成的板状伪足与对照组相比明显减少,相同时间内发生定向迁移的实验组细胞数为0.87±0.21,空白对照组为1.75±0.28,阴性对照组为1.63±0.39,前者与后两者比较差异有统计学意义(P<0.05).结论 PRL-3 siRNA能有效沉默PRL-3基因,并且使异位子宫内膜间质细胞的迁移能力下降.
目的 通過siRNA體外沉默肝再生燐痠酶-3(PRL-3),探討其對異位子宮內膜間質細胞遷移的影響.方法 原代培養異位子宮內膜間質細胞,運用siRNA技術榦擾沉默PRL-3基因,Western blot檢測榦擾後PRL-3蛋白錶達變化,觀察細胞闆狀偽足的形成,採用劃痕實驗、transwell細胞遷移實驗觀察併比較細胞遷移能力的改變.結果 經siRNA 榦擾沉默後實驗組PRL-3蛋白相對錶達量較對照組明顯下降(P<0.05),細胞形成的闆狀偽足與對照組相比明顯減少,相同時間內髮生定嚮遷移的實驗組細胞數為0.87±0.21,空白對照組為1.75±0.28,陰性對照組為1.63±0.39,前者與後兩者比較差異有統計學意義(P<0.05).結論 PRL-3 siRNA能有效沉默PRL-3基因,併且使異位子宮內膜間質細胞的遷移能力下降.
목적 통과siRNA체외침묵간재생린산매-3(PRL-3),탐토기대이위자궁내막간질세포천이적영향.방법 원대배양이위자궁내막간질세포,운용siRNA기술간우침묵PRL-3기인,Western blot검측간우후PRL-3단백표체변화,관찰세포판상위족적형성,채용화흔실험、transwell세포천이실험관찰병비교세포천이능력적개변.결과 경siRNA 간우침묵후실험조PRL-3단백상대표체량교대조조명현하강(P<0.05),세포형성적판상위족여대조조상비명현감소,상동시간내발생정향천이적실험조세포수위0.87±0.21,공백대조조위1.75±0.28,음성대조조위1.63±0.39,전자여후량자비교차이유통계학의의(P<0.05).결론 PRL-3 siRNA능유효침묵PRL-3기인,병차사이위자궁내막간질세포적천이능력하강.
Objective To evaluate the effects of PRL-3 siRNA (small interfering RNA) on the migration of endometriotic stromal cells.Methods Primary endometriotic stromal cells were cultured in vitro.Then PRL-3 (phosphatase of regenerating liver-3) siRNA was transfected to silence the PRL-3 gene.And the PRL-3 protein expression was analyzed by Western blot.The changes of cell migration were detected by cell pseudopod formation, scratch test and transwell cell migration test.Results The expression of PRL-3 protein significantly decreased in the experimental group versus two other control groups (P<0.05).The formation of cell pseudopod was much less in experimental group than that in control groups. Within the same time, the number of migration cells was 0.87±0.21 in experimental group.It was less than 1.75±0.28 in blank control group and 1.63±0.39 in negative control group(P<0.05).Conclusion PRL-3 siRNA can down-regulate the PRL-3 gene and decrease the migratory capacity of endometriotic stromal cells.And PRL-3 may be a promising target in the therapeutics of endometriosis.
