CAJ | 학술논문

目的探讨血管内皮生长因子(VEGF) DNA质粒对胶原-磺化羧甲基壳聚糖真皮支架在猪Ⅲ度烧伤创面修复中血管化的影响。方法将单纯胶原-磺化羧甲基壳聚糖（A组）和负载VEGF IDNA质粒（B绀）胶原-磺化羧甲基壳聚糖两种不同真皮支架各移植于6只猪Ⅲ度烧伤切痂后创面，对植入支架后1、2、3周的创面及植入支架2周创面加植表皮后2周（植入支架后4周）的创面修复情况及病理切片进行观察。同时，通过采用免疫组织化学方法，对1、2、3、4周的支架及创面中表达CD31的新生血管、表达α-SMA的成熟血管和VEGF表达阳性的细胞数进行检测和观察。实验以不植入真皮支架（C组）的烧伤切痂后创面作为对照。结果 A组和B组1～3周创面和2周创面植表皮后2周CD31表达的新生血管分别为18.7±3.1、25.7±2.3、36.8±2.5和26.2±2.9，24.5±3.8、32.3±2.8、39.2±2.2和27.3±3.0；αt-SMA表达的成熟血管分别为11.7±1.9、20.5±1.9、35.0±4.5和24.0±2.8,20.2±3.1、33.5±3.7、38.2±4.5和26.5±2.3；VEGF表达阳性的细胞数分别为48.7±7.9、141.7 ±9.1、201.5±8.6和107.0±8.2,97.3±7.9、172.3±8.1、208.7±8.3和114.0±5.8;C组1～4周创面CD31、α-SMA和VEGF的表达强度分别为6.0±2.0、9.8±3.4、19.3±2.5、18.7±2.2,3.7±2.0、8.7±1.8、13.0±2.5、14.0±2.8,3.5±2.3、10.3±3.5、23.0±5.6、21.5±5.1。A、B、C3组差异均有统计学意义（均P＜0.05）。结论外源性pDNA-VEGF导人胶原-磺化羧甲基壳聚糖真皮支架可促进创面肉芽组织表达更多的VEGF，加快支架血管化及创面修复。
목적 탐토혈관내피생장인자(VEGF) DNA질립대효원-광화최갑기각취당진피지가재저Ⅲ도소상창면수복중혈관화적영향。방법 장단순효원-광화최갑기각취당（A조）화부재VEGF IDNA질립（B감）효원-광화최갑기각취당량충불동진피지가각이식우6지저Ⅲ도소상절가후창면，대식입지가후1、2、3주적창면급식입지가2주창면가식표피후2주（식입지가후4주）적창면수복정황급병리절편진행관찰。동시，통과채용면역조직화학방법，대1、2、3、4주적지가급창면중표체CD31적신생혈관、표체α-SMA적성숙혈관화VEGF표체양성적세포수진행검측화관찰。실험이불식입진피지가（C조）적소상절가후창면작위대조。결과 A조화B조1～3주창면화2주창면식표피후2주CD31표체적신생혈관분별위18.7±3.1、25.7±2.3、36.8±2.5화26.2±2.9，24.5±3.8、32.3±2.8、39.2±2.2화27.3±3.0；αt-SMA표체적성숙혈관분별위11.7±1.9、20.5±1.9、35.0±4.5화24.0±2.8,20.2±3.1、33.5±3.7、38.2±4.5화26.5±2.3；VEGF표체양성적세포수분별위48.7±7.9、141.7 ±9.1、201.5±8.6화107.0±8.2,97.3±7.9、172.3±8.1、208.7±8.3화114.0±5.8;C조1～4주창면CD31、α-SMA화VEGF적표체강도분별위6.0±2.0、9.8±3.4、19.3±2.5、18.7±2.2,3.7±2.0、8.7±1.8、13.0±2.5、14.0±2.8,3.5±2.3、10.3±3.5、23.0±5.6、21.5±5.1。A、B、C3조차이균유통계학의의（균P＜0.05）。결론 외원성pDNA-VEGF도인효원-광화최갑기각취당진피지가가촉진창면육아조직표체경다적VEGF，가쾌지가혈관화급창면수복。
ObjectiveTo investigate the effects of vascular endothelial growth factor (VEGF) DNA plasmids, encoded in collagen-sulfonated carboxymethyl chitosan porous scaffold, on the angiogenesis in fullthickness burn wounds. MethodsWounds and pathological changes were observed at Week 1,2, 3 and 4after pure collagen-sulfonated carboxymethyl chitosan porous scaffold (group A ) and collagen-sulfonated carboxymethyl chitosan porous scaffold encoding VEGF DNA plasmids (group B ) were transplanted onto eachar-removed wounds of full-thickness bum in 6 Bama miniature pigs respectively. Wound healing was observed by pathologic slides "after epidermal grafting for 2 weeks ( at Week 4) onto the surface of different dermal scaffolds transplanted on wounds for 2 weeks.At the same time, new-forming vessels expressing CD31 and mature vessels expressing α-SMA (α-smooth muscle actin) and the expression of VEGF in wounds at Week 1, 2, 3 and 4 were detected in situ by immunohistochemical staining. The bum wounds without any transplanted scaffold (group C) were studied as the controls.ResultsWounds with various scaffolds were different from those without any scaffold. Angiogenesis of the group B was better than that of the group A. Neo-forming micro-vessels expressing CD31 in the wounds of group A or B at Week 1, 2, 3and4 were as follows: 18.7 ±3. 1, 25.7 ±2.3, 36.8 ±2.5 & 26.2 ±2.9; 24.5 ±3.8, 32.3 ±2.8,39.2±2.2 & 27.3 ±3.0.Mature vessels expressing α-SMA: 11.7 ±1.9, 20.5 ±1.9, 35.0 ±4.5 &24. 0 ± 2. 8 ; 20. 2 ± 3. 1, 33.5 ± 3.7, 38. 2 ± 4. 5 & 26. 5 ± 2. 3. The expressions of VEGF: 48. 7 ± 7.9,141.7±9.1, 201.5 ±8.6 & 107.0 ±8.2; 97.3 ±7.9, 172.3 ±8.1, 208.7 ±8.3 & 114.0 ±5.8.Expressing intensity of CD31, α-SMA and VEGF in the wounds of group C at Week 1, 2, 3 & 4: 6.0 ±2.0, 9.8±3.4, 19.3±2.5 & 18.7±2.2; 3.7±2.0, 8.7±1.8, 13.0±2.5 & 14.0±2.8; 3.5 ±2.3,10. 3 ± 3.5, 23.0 ± 5.6 & 21.5 ± 5. 1. There were significant statistical differences among three groups.ConclusionArtificial dermal scaffold encoding exogenous pDNA-VEGF can promote a quicker angiogenesis through a high expression of VEGF. Thus a full-thickness bum wound may be better repaired.