中华心血管病杂志
中華心血管病雜誌
중화심혈관병잡지
Chinese Journal of Cardiology
2008年
3期
205-208
,共4页
猝死,心脏%冠状动脉硬化%免疫组织化学%Toll样受体4
猝死,心髒%冠狀動脈硬化%免疫組織化學%Toll樣受體4
졸사,심장%관상동맥경화%면역조직화학%Toll양수체4
Death,sudden,cardiac%Coronary arteriosclerosis%Immunohistology%Toll-like receptor 4
目的 探讨Toll样受体4(TLR4)在冠状动脉粥样硬化斑块中的表达及其与冠心病猝死的关系.方法 从本教研室2003-2006年尸检档案中挑选病例及心脏标本、蜡块共75例.分为冠心病猝死组(28例)、冠心病非猝死组(简称冠心病组,28例)和对照组(无明显冠状动脉粥样硬化者,19例).用免疫组化SABC法、图像分析技术和SPSS14.0统计学软件检测TLR4在不同组的冠状动脉粥样硬化斑块内表达的阳性细胞面积/扫描面积(R)和平均光密度值(A)及各组间的差异.结果 猝死组中13例(46.4%)TLR4蛋白强阳性表达,11例(39.3%)为较强阳性表达,4例(14.3%)为较弱阳性表达.冠心病组中8例(28.6%)呈较弱的阳性表达,17例(60.7%)很微弱的阳性表达,3例(10.7%)未见阳性表达.对照组冠状动脉内膜及轻度增厚处呈阴性表达.猝死组冠状动脉粥样硬化斑块中TLR4表达的A值(1.140±0.101)和R值(0.0269±0.0027)均高于冠心病组(A值:0.719±0.205,R值:0.0085±0.0007;均P＜0.01)和对照组(A值:0.481 ±0.033,R值:0.0046±0.0004;均P＜0.01).冠心病组的A值也高于对照组(P＜0.05),而R值差异无统计学意义.结论 冠状动脉粥样硬化斑块中TLR4表达情况可作为冠心病猝死者病理诊断的一个重要指标,也可为临床防治冠心病和急性冠状动脉综合征提供一个新的思路.
目的 探討Toll樣受體4(TLR4)在冠狀動脈粥樣硬化斑塊中的錶達及其與冠心病猝死的關繫.方法 從本教研室2003-2006年尸檢檔案中挑選病例及心髒標本、蠟塊共75例.分為冠心病猝死組(28例)、冠心病非猝死組(簡稱冠心病組,28例)和對照組(無明顯冠狀動脈粥樣硬化者,19例).用免疫組化SABC法、圖像分析技術和SPSS14.0統計學軟件檢測TLR4在不同組的冠狀動脈粥樣硬化斑塊內錶達的暘性細胞麵積/掃描麵積(R)和平均光密度值(A)及各組間的差異.結果 猝死組中13例(46.4%)TLR4蛋白彊暘性錶達,11例(39.3%)為較彊暘性錶達,4例(14.3%)為較弱暘性錶達.冠心病組中8例(28.6%)呈較弱的暘性錶達,17例(60.7%)很微弱的暘性錶達,3例(10.7%)未見暘性錶達.對照組冠狀動脈內膜及輕度增厚處呈陰性錶達.猝死組冠狀動脈粥樣硬化斑塊中TLR4錶達的A值(1.140±0.101)和R值(0.0269±0.0027)均高于冠心病組(A值:0.719±0.205,R值:0.0085±0.0007;均P＜0.01)和對照組(A值:0.481 ±0.033,R值:0.0046±0.0004;均P＜0.01).冠心病組的A值也高于對照組(P＜0.05),而R值差異無統計學意義.結論 冠狀動脈粥樣硬化斑塊中TLR4錶達情況可作為冠心病猝死者病理診斷的一箇重要指標,也可為臨床防治冠心病和急性冠狀動脈綜閤徵提供一箇新的思路.
목적 탐토Toll양수체4(TLR4)재관상동맥죽양경화반괴중적표체급기여관심병졸사적관계.방법 종본교연실2003-2006년시검당안중도선병례급심장표본、사괴공75례.분위관심병졸사조(28례)、관심병비졸사조(간칭관심병조,28례)화대조조(무명현관상동맥죽양경화자,19례).용면역조화SABC법、도상분석기술화SPSS14.0통계학연건검측TLR4재불동조적관상동맥죽양경화반괴내표체적양성세포면적/소묘면적(R)화평균광밀도치(A)급각조간적차이.결과 졸사조중13례(46.4%)TLR4단백강양성표체,11례(39.3%)위교강양성표체,4례(14.3%)위교약양성표체.관심병조중8례(28.6%)정교약적양성표체,17례(60.7%)흔미약적양성표체,3례(10.7%)미견양성표체.대조조관상동맥내막급경도증후처정음성표체.졸사조관상동맥죽양경화반괴중TLR4표체적A치(1.140±0.101)화R치(0.0269±0.0027)균고우관심병조(A치:0.719±0.205,R치:0.0085±0.0007;균P＜0.01)화대조조(A치:0.481 ±0.033,R치:0.0046±0.0004;균P＜0.01).관심병조적A치야고우대조조(P＜0.05),이R치차이무통계학의의.결론 관상동맥죽양경화반괴중TLR4표체정황가작위관심병졸사자병리진단적일개중요지표,야가위림상방치관심병화급성관상동맥종합정제공일개신적사로.
Objective To investigate the Toll like receptor 4(TLR4)expression in the coronary atheroselerotic plaques in patients died from sudden cardiac death(SCD)or non SCD.Methods Autopsied coronary artery samples from 75 patients died from SCD(n=28),non-SCD(n=28)or non-CHD(n=19)were examined and the R value(positive cells'areas/scanning areas)and A value(average optical density)of TLR4 expression in the coronary arteries were detected qualitatively by the immunohistochemistry(SABC method)and image analysis technologies.Results SCD group:13(46.4%)cases showed strong positive expression of TLR4;11(39.3%),positive expression;4(14.3%),weak positive expression.CHD group:8(28.6%)cases showed weak positive expression;17(60.7%),very weak positive expression;3 (10.7%),no positive expression.There was no positive expression of TLR4 in non-CHD samples.A (1.140±0.101)and R value(0.0269±0.0027)in SCD group were significantly higher than in non-SCD and control groups(all P＜0.01).A value was siginificantly higher in CHD group(0.719±0.205)than that in control group(0.481±0.033,P＜0.05)while R value(0.0085±0.0007,0.0046±0.0004)was similar between the groups(P＞0.05).Conclusion The increased positive expressive of TLR4 in the atherosclerotic plaque can be regarded as an important pathological marker of SCD.
