CAJ | 학술논문

目的为研究与人前列腺癌细胞(PC-3M)侵袭能力相关的靶分子.方法采用有限稀释法分离单克隆细胞株,并应用单层细胞侵袭等实验鉴定各亚系的体外侵袭能力;借助RT-PCR和免疫组化的方法,分别在转录和翻译水平检测5株侵袭能力不同的PC-3M亚系尿激酶型纤溶酶原激活物受体(u-PAR)的表达.结果高侵袭亚系u-PAR基因mRNA的表达和蛋白质水平均明显高于低侵袭亚系.结论PC-3M亚系u-PAR的高表达与其较强的侵袭能力密切相关,而u-PAR可能是抑制高侵袭亚系侵袭效应的一个重要靶分子.
목적위연구여인전렬선암세포(PC-3M)침습능력상관적파분자.방법채용유한희석법분리단극륭세포주,병응용단층세포침습등실험감정각아계적체외침습능력;차조RT-PCR화면역조화적방법,분별재전록화번역수평검측5주침습능력불동적PC-3M아계뇨격매형섬용매원격활물수체(u-PAR)적표체.결과고침습아계u-PAR기인mRNA적표체화단백질수평균명현고우저침습아계.결론PC-3M아계u-PAR적고표체여기교강적침습능력밀절상관,이u-PAR가능시억제고침습아계침습효응적일개중요파분자.
Objective: To select a target molecule associated with invasive potential in PC-3M cell. Methods: Cell subclones were isolated from PC-3M cell line with the method of limited dilution and their invasive ability characterized by monolayer invasion assay. The expression of u-PAR in the cell subclones at mRNA and protein levels was assayed respectively by non-competitive quantitative RT-PCR and immunohistochemical assay. Results: The expression level of u-PAR in highly invasive cell subclones was higher than that of lower invasive subclones. Conclusion: The higher expression level of u-PAR is associated with the relative strong invasive ability of PC-3M subclones. It is indicated that the u-PAR might be a promising target molecule for inhibiting invasion of highly invasive PC-3M cell subclones.