CAJ | 학술논문

根据珊瑚藻(Corallina afficinalis L.)R-藻红蛋白γ亚基N末端部分氨基酸序列(P83592)设计简并引物,结合RACE方法,扩增获得g亚基的全长cDNA序列.结果表明,序列全长为2 308 bp(AY209894),5'非编码区长1 203bp,3'非编码区长145 bp,编码区长960 bp,编码320个氨基酸组成的前体,包含71个氨基酸构成的信号肽和249个氨基酸组成的成熟蛋白.成熟蛋白序列内部存在重复序列与前人的报道一致.珊瑚藻亚基cDNA序列不同克隆子的测序结果表明,g亚基cDNA序列存在不同的3'末端,说明该基因可能存在多个拷贝或存在转录后加工.此外,扩增获得g亚基DNA序列(AY308999),比较表明编码区内部没有内含子存在.本文是对珊瑚藻R-藻红蛋白g亚基基因序列的首次报道.
근거산호조(Corallina afficinalis L.)R-조홍단백γ아기N말단부분안기산서렬(P83592)설계간병인물,결합RACE방법,확증획득g아기적전장cDNA서렬.결과표명,서렬전장위2 308 bp(AY209894),5'비편마구장1 203bp,3'비편마구장145 bp,편마구장960 bp,편마320개안기산조성적전체,포함71개안기산구성적신호태화249개안기산조성적성숙단백.성숙단백서렬내부존재중복서렬여전인적보도일치.산호조아기cDNA서렬불동극륭자적측서결과표명,g아기cDNA서렬존재불동적3'말단,설명해기인가능존재다개고패혹존재전록후가공.차외,확증획득g아기DNA서렬(AY308999),비교표명편마구내부몰유내함자존재.본문시대산호조R-조홍단백g아기기인서렬적수차보도.
The full-length cDNA of the γ subunit of R-phycoerythrin from Corallina officinalis L. was cloned by rapid amplification of cDNA ends (RACE) method, and sequenced. The full-length cDNA is a 2 308 bp consisting of 5′ untranslated region (UTR) of 1 203 bp, an open reading frame (ORF) of 960 bp that encodes 320 amino acids, and 3′ UTR of 145 bp. The mature γ polypeptide contains two unique internal repeat domains as reported by Apt et al. (2001). Sequence analysis of the different clones revealed different 3′-end sequences at the γ subunit. The difference between the 3′-end sequences suggests that the γ subunit may have more than one copy, or have gone through different post-transcriptional modification. By comparing the DNA and cDNA sequences, we found that the γ subunit is an intronless gene. This is the first report of the γ subunit gene of R-phycoerythrin from C. officinalis.