CAJ | 학술논문

Tcmporins是从蛙属中得到的一类羧基端酰胺化的疏水性抗菌肽,具有抗细菌、霉菌、酵母菌、原虫及病毒活性.为丫研究牛蛙皮肤抗菌肽的多样性及其结构特点,根据GenBank数据库中蛙属抗菌肽基因信号肽序列设计简并引物,从牛蛙皮肤cDNA文库中克隆到两个新的temporins家族抗菌肽,命名为mmporin-La(LLRHVVKILEKUYLarnide)和temporin-Lb (LFRHVVKIFEKYLarnide).合成的temporin-La和temporin-Lb肽具有很强的抗菌活性,尤其是对革兰氏阳性细菌.溶血性测定结果表明,temporin-La浓度高至250mg/L时对兔红细胞仍无溶血活性,而tcmporin-Lb具有较弱的溶血活性(半数致死浓度LC50≈230 μmol/L).通过透射电镜观察temporin-La和temporin-Lb处理过的金黄色葡萄球菌的细胞结构,发现它们都能直接地杀死细菌,但作用机制不一样.
Tcmporins시종와속중득도적일류최기단선알화적소수성항균태,구유항세균、매균、효모균、원충급병독활성.위아연구우와피부항균태적다양성급기결구특점,근거GenBank수거고중와속항균태기인신호태서렬설계간병인물,종우와피부cDNA문고중극륭도량개신적temporins가족항균태,명명위mmporin-La(LLRHVVKILEKUYLarnide)화temporin-Lb (LFRHVVKIFEKYLarnide).합성적temporin-La화temporin-Lb태구유흔강적항균활성,우기시대혁란씨양성세균.용혈성측정결과표명,temporin-La농도고지250mg/L시대토홍세포잉무용혈활성,이tcmporin-Lb구유교약적용혈활성(반수치사농도LC50≈230 μmol/L).통과투사전경관찰temporin-La화temporin-Lb처리과적금황색포도구균적세포결구,발현타문도능직접지살사세균,단작용궤제불일양.
Temporins are a kind of small,hydrophobic and C-terminus amidated antimicrobial peptides from Rana species.They are effective against bacteria,fungi,yeast,protozoa and viruses.Two novel temporins named as temporin-La(LLRHWKILEKYLanifc) and temporin-Lb(LFRHVVKIFEK.Ylamid.) were cloned from Lithobates catesbeianus.Synthetic peptides of temporin-La and temporin-Lb showed strong antimicrobial activities against bacteria tested,especially Gram-positive bacteria.Besides,temporin-La showed no haemolytic activity to rabbit erythrocytes at the concentration of 250 mg/L while temporin-Lb showed weak haemolytic activity(LC50≈ 230 μmol/L).Transmission electron microscopy showed that temporin-La and temporin-Lb induced different effects on bacterial structure of Staphylococcus aureus.