CAJ | 학술논문

目的:探讨细胞周期蛋白酶抑制剂flavopiridol(FP)对耐阿霉素尤文肉瘤细胞增殖抑制及凋亡诱导的作用机制.方法:采用MTT法测定阿霉素(ADM)及falvopiridol对VH-64/ADR细胞的半数抑制浓度(IC50),计算耐药倍数;流式细胞计数仪(FCM)检测falvopiridol给药后VH-64/ADR细胞周期的变化;Western-blotting方法检测细胞中Bcl-2、pro-caspase-3、活化型多聚ADP核糖多聚酶(PARP-85)蛋白的表达.结果:flavopiridol可抑制尤文肉瘤耐阿霉素细胞株VH/ADR的细胞增殖,其效果呈时间及浓度相关性(P<0.05).flavopiridol给药后,耐药细胞株VH-64/ADR的细胞凋亡率明显高于对照组(P<0.05);bcl-2、pro-casepase-3表达下调,而活性型PARP表达上调. 结论:FP可有效诱导VH-64/ADR细胞发生凋亡,其机制可能与线粒体信号传导途径有关.
목적:탐토세포주기단백매억제제flavopiridol(FP)대내아매소우문육류세포증식억제급조망유도적작용궤제.방법:채용MTT법측정아매소(ADM)급falvopiridol대VH-64/ADR세포적반수억제농도(IC50),계산내약배수;류식세포계수의(FCM)검측falvopiridol급약후VH-64/ADR세포주기적변화;Western-blotting방법검측세포중Bcl-2、pro-caspase-3、활화형다취ADP핵당다취매(PARP-85)단백적표체.결과:flavopiridol가억제우문육류내아매소세포주VH/ADR적세포증식,기효과정시간급농도상관성(P<0.05).flavopiridol급약후,내약세포주VH-64/ADR적세포조망솔명현고우대조조(P<0.05);bcl-2、pro-casepase-3표체하조,이활성형PARP표체상조. 결론:FP가유효유도VH-64/ADR세포발생조망,기궤제가능여선립체신호전도도경유관.
Objective:To investigate the growth-inhibitory and apoptosis inducing effect of flavopiridol on human Ewing Sarcoma VH-64/ADR cell and its mechanism in vitro. Methods: The proliferation of VH-64 and VH-64/ADR cells was determined by using MTT method.Cell cycle progression and apoptosis ratio were determined by flow cytometry.The expression of bcl-2,cleaved-PARP,casepase-3 were detected by western blot. Results:After exposure to flavopiridol,the growth of VH-64/ADR cells were inhibited in a both time-dependent and dose-dependent manner(P<0.05).Apoptosis rate of VH-64/ADR was higher than that of control group(P<0.05).The expression of bcl-2 and pro-casepase-3 were down-regulated,and the expression of active-form PARP was up-regulated. Conclusion: The apoptosis of VH-64/ADR cells induced by flavopiridol might be via mitochondrial pathway.