CAJ | 학술논문

目的建立体外非接触共培养模型,诱导小鼠骨髓间充质干细胞(MSCs)向肺泡上皮细胞的分化.方法分三组,每组6例.对照组:小鼠MSCs单独培养组;实验组A:正常肺组织单细胞悬液+MSCs;实验组B:损伤肺组织单细胞悬液+MSCs.共同培养8d,激光共聚焦和RT-PCR检测共培养体系下室中的SP-C和AQP5的表达情况.结果对照组和实验组A都只检测到AQP5;而实验组B可同时检测到AQP5和SP-C.实验组B的AQP5 mRNA的表达量较对照组明显增多(P<0.01),而与实验组A比较,其AQP5 mRNA的表达量也有统计学差异(P<0.01).但是,实验组A与对照组比较则无明显统计学差异(P>0.05).结论本实验室成功建立了体外非接触诱导小鼠MSCs分化为肺泡上皮细胞的实验模型.
목적 건입체외비접촉공배양모형,유도소서골수간충질간세포(MSCs)향폐포상피세포적분화.방법 분삼조,매조6례.대조조:소서MSCs단독배양조;실험조A:정상폐조직단세포현액+MSCs;실험조B:손상폐조직단세포현액+MSCs.공동배양8d,격광공취초화RT-PCR검측공배양체계하실중적SP-C화AQP5적표체정황.결과 대조조화실험조A도지검측도AQP5;이실험조B가동시검측도AQP5화SP-C.실험조B적AQP5 mRNA적표체량교대조조명현증다(P<0.01),이여실험조A비교,기AQP5 mRNA적표체량야유통계학차이(P<0.01).단시,실험조A여대조조비교칙무명현통계학차이(P>0.05).결론 본실험실성공건립료체외비접촉유도소서MSCs분화위폐포상피세포적실험모형.
Objective To establish the co-culture model in vitro and induce bone marrow mesenchymal stem cells (MSCs) to differentiate into lung alveolar epithelial cells. Methods Each group had 6 samples, control group was MSCs alone; Group A was the MSCs cultured with the cells from normal lung; and Group B was the MSCs with the cells from injuried lung. Each group was cultured for 8 days and the two markers of lung alveolar epithelial cells including AQP5 and SP-C were tested by laser confocal microscopy and RT-PCR. Results Only AQP5 was detected in the control group and Group A, both AQP5 and SP-C were detected in Group B, the AQP5 mRNA expression in Group B was significantly increased compared with that in the control group(P<0.01). The AQP5 mRNA expression in Group B was also significantly increased compared with that in Group A (P<0.01). But there was no significant difference in AQP5 mRNA expression between Group A and control group. Conclusion We have successfully established the co-culture model in vitro to induce bone marrow mesenchymal stem cells to differentiate into lung epithelial cells.