CAJ | 학술논문

对由pCB6.3kb、pCB5.3kb和pZH01-2.72kb三个不同表达载体转化的转稻瘟病抗性基因Pi-d2水稻株系进行稻瘟病抗性分析.结果表明,转Pi-d2基因水稻的9个高代株系对来自四川的39个稻瘟病菌株表现不同的抗性,抗病频率最高达91.7%;4个转基因早代纯合株系对来自中国农业科学院的58个菌株中的81.48%以上菌株表现抗性,具有广谱抗性特点.稻瘟病菌粗毒素筛选结果表明,来自转基因植株的幼胚愈伤组织诱导率随培养基中粗毒素浓度提高而降低,粗毒素浓度达到40%时,幼胚愈伤诱导率为49.33%,受体对照幼胚愈伤组织诱导率为5%.田间诱发条件下,转基因株系在大田的穗瘟发病率为0%～50%, 抗性较受体对照大幅度提高.
대유pCB6.3kb、pCB5.3kb화pZH01-2.72kb삼개불동표체재체전화적전도온병항성기인Pi-d2수도주계진행도온병항성분석.결과표명,전Pi-d2기인수도적9개고대주계대래자사천적39개도온병균주표현불동적항성,항병빈솔최고체91.7%;4개전기인조대순합주계대래자중국농업과학원적58개균주중적81.48%이상균주표현항성,구유엄보항성특점.도온병균조독소사선결과표명,래자전기인식주적유배유상조직유도솔수배양기중조독소농도제고이강저,조독소농도체도40%시,유배유상유도솔위49.33%,수체대조유배유상조직유도솔위5%.전간유발조건하,전기인주계재대전적수온발병솔위0%～50%, 항성교수체대조대폭도제고.
Transgenic rice lines harboring Pi-d2 gene under the control of different promoters from three expression vector pCB6.3kb, pCB5.3kb and pZH01-2.72kb were tested for resistance to Magnaporthe grisea. The results showed that 9 stably transgenic lines had different resistances to 39 strains of M. grisea from Sichuan Province, China, and the highest disease-resistance frequency was 91.7%. Four early homozygous transgenic lines of Pi-d2 gene exhibited resistance to more than 81.48% of 58 strains from Chinese Academy of Agricultural Sciences, which indicates that the transgenic rice lines possessed wide-spectrum resistance against various rice blast races. The results of transgenic embryonic callus selected by crude toxin showed that the induction rate of embryonic callus reduced by increased concentration of toxin in medium. When the toxin concentration was 40% in medium, the induction rate of embryonic callus of transgenic plant was 49.33%, while that of the receptor control was 5%. The disease incidence of neck blast were 0% to 50% in the transgenic lines under induction of rice blast disease in fields, showing the higher resistance to rice blast than that of receptor control.