CAJ | 학술논문

通过菌种筛选、摇瓶发酵条件优化,系统地研究了微生物发酵生产豆粕生物肽的工艺.结果表明:枯草芽胞杆菌为最佳菌株,其豆粕生物肽最佳生产工艺条件为:种子培养时间16 h,豆粕粒度40目,初始pH6.8,葡萄糖2%,豆粕11%,接种量6%,发酵时间48 h;在此工艺条件下蛋白质降解为多肽的得率为41.18%.
통과균충사선、요병발효조건우화,계통지연구료미생물발효생산두박생물태적공예.결과표명:고초아포간균위최가균주,기두박생물태최가생산공예조건위:충자배양시간16 h,두박립도40목,초시pH6.8,포도당2%,두박11%,접충량6%,발효시간48 h;재차공예조건하단백질강해위다태적득솔위41.18%.
In this paper,the production method of using bacterium fermenting soybean meal through selecting strain,optimizing the condition in the spinner flask was studied.Taking the content of α-N in the Fermentation Broth as an index,the Aspergillus niger,Aspergillus oryzae and Bacillus subtilis was selected and the Bacillus subtilis was the optimum strain.The processing technology of the biologic peptide of soybean meal was determined as follows:seed inoculation time 16 h,granularity of the soybean meal 40 holes,pH 6.8,glucose 2%,soybean meal 11%,inoculation amount 6%,fermentation time 48h.Under the optimum technological conditions,the ratio of the peptides from the soybean protein was 41.18%.