CAJ | 학술논문

为研究奶牛性控冻精人工授精效果并合理制定人工授精方案,采用流式细胞仪制备性控冻精,对3 947头健康荷斯坦牛进行人工授精.结果表明:①性控冻精和常规冻精的受胎率、产犊率差异不显著(P＞0.05),但性控冻精的雌犊率和繁殖率分别达到92.01%和50.03%,对照组仅为51.13%和34.97%,差异极显著(P＜0.01).②青年牛的情期受胎率达到55.25%,极显著高于经产牛(P＜0.01);青年牛的产犊率达到98.31%,显著高于经产牛(P＜0.05);青年牛的繁殖率为51.84%,经产牛为42.12%,存在极显著差异(P＜0.01).③采用第1次人工授精后10～11 h重复授精1次,每次1支性控冻精的授精方案,其情期受胎率为55.17%,繁殖率达到55.81%,同其他方案相比,差异极显著(P＜0.01).应用性控冻精进行人工授精可以显著提高母牛的繁殖效率.
위연구내우성공동정인공수정효과병합리제정인공수정방안,채용류식세포의제비성공동정,대3 947두건강하사탄우진행인공수정.결과표명:①성공동정화상규동정적수태솔、산독솔차이불현저(P＞0.05),단성공동정적자독솔화번식솔분별체도92.01%화50.03%,대조조부위51.13%화34.97%,차이겁현저(P＜0.01).②청년우적정기수태솔체도55.25%,겁현저고우경산우(P＜0.01);청년우적산독솔체도98.31%,현저고우경산우(P＜0.05);청년우적번식솔위51.84%,경산우위42.12%,존재겁현저차이(P＜0.01).③채용제1차인공수정후10～11 h중복수정1차,매차1지성공동정적수정방안,기정기수태솔위55.17%,번식솔체도55.81%,동기타방안상비,차이겁현저(P＜0.01).응용성공동정진행인공수정가이현저제고모우적번식효솔.
To evaluate the effect of artificial insemination with frozen-sexed sperm on the reproductive performance of dairy cow,and to further improve insemination strategies,frozen spermwassexed by flow cytometry. Then 3 947 health Holstein cow were artificially inseminated with frozen sperm unsexed or sexed. The results showed:①The pregnancy and calving rates were 48.91% and 97.97% for sexed-sperm AIs, 51.74% and 98.05% for the unsexed-sperm AIs(P＞0.05).The proportion of female calves derived from sexed-sperm AIs was 92.01% compared with 51.13% for control AIs (P<0.01), the reproductive rate was 51.13% for sexed-sperm AIs and 34.97% for control AIs(P<0.01).② The pregnancy rate was 55.25% for primiparous cow and was obviously higher than that of multiparous cow (P<0.01), the calving rate was 98.31% for primiparous cow and was higher than that of multiparous cow(P<0.05), the reprodcutive rates was 51.84% for primiparous cow and 42.12% for multiparous cow, there were significant differences (P<0.01).③When a insemination was followed by a second one 10-11 hours later, and a single-doses sperm was used in each time, the pregnancy and reproductive rates were both the highest (P<0 01).Our results indicated that AI using sexed sperm under field conditions in commercial dairy herds without oestrus synchronization resulted in a significantly improvement of reprocuctive effect compared with AI using unsexed sperm.