四川大学学报（医学版）
四川大學學報（醫學版）
사천대학학보（의학판）
JOURNAL OF SICHUAN UNIVERSITY(MEDICAL SCIENCE EDITION)
2010年
2期
212-217
,共6页
孙万森%任艳芸%赵艳龙%马巧亚
孫萬森%任豔蕓%趙豔龍%馬巧亞
손만삼%임염예%조염룡%마교아
阿霉素肾病%蛋白尿%硫酸肝素蛋白多糖%祛风通络方
阿黴素腎病%蛋白尿%硫痠肝素蛋白多糖%祛風通絡方
아매소신병%단백뇨%류산간소단백다당%거풍통락방
Adriamycin nephropathy%Proteinuria%Heparan sulfate protioglycan Qufengtongluo recipe
目的 观察阿霉素肾病大鼠肾小球基底膜硫酸肝素蛋白多糖(HSPG)蛋白及其mRNA表达及祛风通络方对其表达的影响.方法 尾静脉注射阿霉素建立阿霉素肾病大鼠模型,并随机分为A(空白对照,n=32)、B(模型,n=18)、C(祛风通络方,n=18)、D(祛风通络方+激素,n=18)、E(激素,n=18)、F(苯那普利,n=18)组,应用间接免疫荧光染色、RT-PCR分别检测各组大鼠肾小球基底膜HSPG的荧光分布和HSPG mRNA表达的变化.结果 造模成功后透射电镜示B组肾小球足突呈部分或弥漫性融合.干预结束后与B组相比,C、E组HSPG的荧光分布呈连续性或斑片状,平均荧光强度明显增强(P<0.01);D、F组HSPG的荧光呈不连续分布或点状分布,平均荧光强度增强(P<0.01).干预结束后与B组相比,各干预组HSPG mRNA的表达均上调(P<0.01),尤以C、F组明显.相关分析显示24 h尿蛋白定量与HSPG蛋白表达呈明显负相关(r=-0.7353,P<0.001).结论 肾小球基底膜HSPG的表达异常和分布改变是阿霉素肾病大鼠蛋白尿发生发展的重要机制,祛风通络方可能通过改变HSPG的表达和分布而达到治疗蛋白尿的目的 .
目的 觀察阿黴素腎病大鼠腎小毬基底膜硫痠肝素蛋白多糖(HSPG)蛋白及其mRNA錶達及祛風通絡方對其錶達的影響.方法 尾靜脈註射阿黴素建立阿黴素腎病大鼠模型,併隨機分為A(空白對照,n=32)、B(模型,n=18)、C(祛風通絡方,n=18)、D(祛風通絡方+激素,n=18)、E(激素,n=18)、F(苯那普利,n=18)組,應用間接免疫熒光染色、RT-PCR分彆檢測各組大鼠腎小毬基底膜HSPG的熒光分佈和HSPG mRNA錶達的變化.結果 造模成功後透射電鏡示B組腎小毬足突呈部分或瀰漫性融閤.榦預結束後與B組相比,C、E組HSPG的熒光分佈呈連續性或斑片狀,平均熒光彊度明顯增彊(P<0.01);D、F組HSPG的熒光呈不連續分佈或點狀分佈,平均熒光彊度增彊(P<0.01).榦預結束後與B組相比,各榦預組HSPG mRNA的錶達均上調(P<0.01),尤以C、F組明顯.相關分析顯示24 h尿蛋白定量與HSPG蛋白錶達呈明顯負相關(r=-0.7353,P<0.001).結論 腎小毬基底膜HSPG的錶達異常和分佈改變是阿黴素腎病大鼠蛋白尿髮生髮展的重要機製,祛風通絡方可能通過改變HSPG的錶達和分佈而達到治療蛋白尿的目的 .
목적 관찰아매소신병대서신소구기저막류산간소단백다당(HSPG)단백급기mRNA표체급거풍통락방대기표체적영향.방법 미정맥주사아매소건립아매소신병대서모형,병수궤분위A(공백대조,n=32)、B(모형,n=18)、C(거풍통락방,n=18)、D(거풍통락방+격소,n=18)、E(격소,n=18)、F(분나보리,n=18)조,응용간접면역형광염색、RT-PCR분별검측각조대서신소구기저막HSPG적형광분포화HSPG mRNA표체적변화.결과 조모성공후투사전경시B조신소구족돌정부분혹미만성융합.간예결속후여B조상비,C、E조HSPG적형광분포정련속성혹반편상,평균형광강도명현증강(P<0.01);D、F조HSPG적형광정불련속분포혹점상분포,평균형광강도증강(P<0.01).간예결속후여B조상비,각간예조HSPG mRNA적표체균상조(P<0.01),우이C、F조명현.상관분석현시24 h뇨단백정량여HSPG단백표체정명현부상관(r=-0.7353,P<0.001).결론 신소구기저막HSPG적표체이상화분포개변시아매소신병대서단백뇨발생발전적중요궤제,거풍통락방가능통과개변HSPG적표체화분포이체도치료단백뇨적목적 .
Objective To investigate the expression of HSPG in glomerular base membrane of adriamycin-induced nephropathy (AN) rats, and the effect of Qufengtongluo recipe on HSPG mRNA expression and proteinuria in AN rats. Methods One hundred forty rats were used in this study, including 32 rats in normal control group. AN was induced in the left rats by a single tail intravenous injection of adriamycin.Three weeks later, 90 AN rats were randomly divided into five groups: the nephropathy group (B, n=18), the Qufeng group(C, n=18), Qufeng and prednisone group (D,n=18), prednisone group(E,n=18) and benazepri group(F,n=18). The rats in these five groups were treated with different combination of Qufeng recipe and prednisone.In each group, renal tissue samples were collected at week 3 and 7. The distribution, expression of HSPG was examined by indirect immunofluorescence, and semi-quantity RT-PCRrespectively Results ① In AN rats, the diffuse fusion and effacement of foot processes were observed when model established. ② Compared with nephropathy group, the average fluorescence intensity of HSPG dramatically increased in Qufeng group and prednisone group (P<0.01), similarly, it also increased in D and F groups (P<0.01). ③ Compared with nephropathy group, the expression of HSPG mRNA was significantly up-regulated in other groups.(P<0.01), especially in C and F groups. There was significant negative correlation between the expression of HSPG and quantity of 24-hour proteinuria. Conclusion The abnormal expression of HSPG and their altered distributions may be an important molecular mechanism that leads to the occurrence and development of proteinuria in AN rats. The effect of Qufengtongluo recipe on nephrotic syndrome might be related to the alteration of HSPG expression and distribution in glomerulus.
