CAJ | 학술논문

目的评价载药敷料体外细胞毒性和释药性.方法按国标GB/T14233.2-2005,规定的MTT法操作,通过光学倒置显微镜观察L929细胞形态和增殖状况定性分析载药敷料体外细胞毒性,通过吸光度(OD)值计算细胞相对增殖率定量分析载药敷料体外细胞毒性等级;以磷酸盐缓冲液(PBS7.4)为溶出介质,(32±5)℃模拟人体表皮温度,于1/6、1/2、1、3、16、24、36和48 h不同时间点取样,采用紫外分光光度法测定载药敷料体外累积释放性能.结果该载药敷料浸提原液中的细胞形态正常,贴壁生长良好,细胞平均相对增殖率(RGR)为91.25%,毒性为1级.载药敷料的释药动力学符合Higuchi方程:拟合方程为M_t/M_∞=0.3271t~(0.239).结论该载药敷料细胞相容性良好,并具有一定的缓释性能.
목적 평개재약부료체외세포독성화석약성.방법 안국표GB/T14233.2-2005,규정적MTT법조작,통과광학도치현미경관찰L929세포형태화증식상황정성분석재약부료체외세포독성,통과흡광도(OD)치계산세포상대증식솔정량분석재약부료체외세포독성등급;이린산염완충액(PBS7.4)위용출개질,(32±5)℃모의인체표피온도,우1/6、1/2、1、3、16、24、36화48 h불동시간점취양,채용자외분광광도법측정재약부료체외루적석방성능.결과 해재약부료침제원액중적세포형태정상,첩벽생장량호,세포평균상대증식솔(RGR)위91.25%,독성위1급.재약부료적석약동역학부합Higuchi방정:의합방정위M_t/M_∞=0.3271t~(0.239).결론 해재약부료세포상용성량호,병구유일정적완석성능.
Objective To evaluate the cellular toxic and release of pharmaceutical dressing.Method Following the State standard GB/T14233.2-2005.the L929 cellular merphology was observed by inveded microscopy after 72h and proliferation of the cells was examined using mitochondrial function(MTT)assay.Relative growth rate (RGR)was calculated and cytotoxicity grade was evaluated by absorbency(OD)data.With PBS7.4 as dissolution media,and(32±0.5)℃as dissolution temperature,the release rate was determined with UV method with the determination wavelength of 288 nm and the dissolved liquid in 1/6,1/2,1,3,16,24,36 and 48 h.Results The average cell RGR of the pharmaceutical dressing is 91.25%and reaches 1 cytotoxicity grade.L929 cellular morphology is normal.Pharmaceutical dressing release accord to Higuchi equation,and the simulated equation is M_t/M_∞=0.3271t~(0.239).Conclusions Biologic compatibility of the pharmaceutical dressing is good,and the release of levofloxacin from the pharmaceutical dressing is sustained in vitro.