CAJ | 학술논문

背景:有研究表明CpG寡脱氧核苷酸可增强外周血单个核细胞的功能,但对1型糖尿病患者的影响至今少有报道.目的:观察CpG寡脱氧核苷酸对1型糖尿患者患者与健康志愿者外周血单个核细胞γ干扰素、白细胞介素12,10表达的影响.方法:将1型糖尿病患者与健康志愿者的外周血单个核细胞根据刺激物不同分为空白对照组、CpG寡脱氧核苷酸组.用RT-PCR法检测外周血单个核细胞γ干扰素、白细胞介素12 mRNA和白细胞介素10 mRNA的表达.结果与结论:1型糖尿患者γ干扰素和白细胞介素12 mRNA的表达明显低于健康志愿者(P < 0.01).1型糖尿患者与健康志愿者外周血单个核细胞经CpG寡脱氧核苷酸组刺激后,γ干扰素和白细胞介素12 mRNA的表达增高(P < 0.01),白细胞介素10 mRNA的表达无差异(P > 0.05).结果提示,CpG寡脱氧核苷酸可促进1型糖尿患者外周血单个核细胞表达γ干扰素和白细胞介素12.
배경:유연구표명CpG과탈양핵감산가증강외주혈단개핵세포적공능,단대1형당뇨병환자적영향지금소유보도.목적:관찰CpG과탈양핵감산대1형당뇨환자환자여건강지원자외주혈단개핵세포γ간우소、백세포개소12,10표체적영향.방법:장1형당뇨병환자여건강지원자적외주혈단개핵세포근거자격물불동분위공백대조조、CpG과탈양핵감산조.용RT-PCR법검측외주혈단개핵세포γ간우소、백세포개소12 mRNA화백세포개소10 mRNA적표체.결과여결론:1형당뇨환자γ간우소화백세포개소12 mRNA적표체명현저우건강지원자(P < 0.01).1형당뇨환자여건강지원자외주혈단개핵세포경CpG과탈양핵감산조자격후,γ간우소화백세포개소12 mRNA적표체증고(P < 0.01),백세포개소10 mRNA적표체무차이(P > 0.05).결과제시,CpG과탈양핵감산가촉진1형당뇨환자외주혈단개핵세포표체γ간우소화백세포개소12.
BACKGROUND: CpG oligonucleotide has been shown to strengthen the function of peripheral blood mononuclear cells (PBMCs), but its effects on type 1 diabetes mellitus has been rarely reported. OBJECTIVE: To investigate the effects of CpG oligonucleotide on the expression of interferon γ (IFN-γ), interleukin (IL) -12 and IL-10 in PBMCs in patients with type 1 diabetes mellitus versus healthy controls. METHODS: PBMCs were isolated from patients with type 1 diabetes mellitus and healthy controls and then cultured in RPMI-1640 with non-stimulator (control group) and CpG oligonucleotide (CpG oligonucleotide group), respectively. The mRNA expression of IFN-γ, IL-10, and IL-12 in PBMCs was detected by reverse transcription-polymerase chain reaction. RESULTS AND CONCLUSION: mRNA expression of IFN-γ and IL-10 was significantly lower in patients with type 1 diametes mellitus than in healthy controls (P < 0.01). In the CpG oligonucleotide group, the mRNA expression of IFN-γand IL-12 was significantly higher than in the healthy control group (P < 0.01), but the mRNA expression of IL-10 was similar to that in the healthy control group (P > 0.05). These findings demonstrated that CpG oligonucleotide can promote the production of IFN-γ and IL-12 in PBMCs of type 1 diametes mellitus.