中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2001年
3期
178-180
,共3页
缪珩%徐家蓉%游娜%于浩泳%汪晓农
繆珩%徐傢蓉%遊娜%于浩泳%汪曉農
무형%서가용%유나%우호영%왕효농
糖尿病%胰腺,移植%膜,人工%动物实验%大鼠
糖尿病%胰腺,移植%膜,人工%動物實驗%大鼠
당뇨병%이선,이식%막,인공%동물실험%대서
目的 通过对同一来源的成猪胰岛采用两种不同的免疫隔离材料进行微囊化包裹，并植入糖尿病大鼠腹腔内加以比较，以明确两种方法间的优劣。方法 将同一来源的成猪胰岛分成三组：未微囊化组、海藻酸钠微囊组和琼脂糖微囊组。24只糖尿病大鼠随机分为4组：对照组、未微囊化胰岛移植组、海藻酸钠微囊化胰岛移植组和琼脂糖微囊化胰岛移植组，分别进行腹腔内胰岛移植。结果 4组糖尿病大鼠胰岛移植前血糖水平无显著差异，移植后1周分别为(22.67±1.15)mmol/L，(18.58±2.62)mmol/L，(12.27±1.36)mmol/L和(12.38±2.68)mmol/L，后2组与前2组相比均有显著差异，且生存期明显长于前2组，但琼脂糖微囊化胰岛组的生存期明显短于海藻酸钠微囊化胰岛组(分别为平均57.10d和145.17d)。结果 两种包膜材料制备的微囊化成猪胰岛均可存活于糖尿病大鼠体内并发挥生物功能，但海藻酸钠微囊化胰岛组较琼脂糖微囊化胰岛组的存活期明显延长。
目的 通過對同一來源的成豬胰島採用兩種不同的免疫隔離材料進行微囊化包裹，併植入糖尿病大鼠腹腔內加以比較，以明確兩種方法間的優劣。方法 將同一來源的成豬胰島分成三組：未微囊化組、海藻痠鈉微囊組和瓊脂糖微囊組。24隻糖尿病大鼠隨機分為4組：對照組、未微囊化胰島移植組、海藻痠鈉微囊化胰島移植組和瓊脂糖微囊化胰島移植組，分彆進行腹腔內胰島移植。結果 4組糖尿病大鼠胰島移植前血糖水平無顯著差異，移植後1週分彆為(22.67±1.15)mmol/L，(18.58±2.62)mmol/L，(12.27±1.36)mmol/L和(12.38±2.68)mmol/L，後2組與前2組相比均有顯著差異，且生存期明顯長于前2組，但瓊脂糖微囊化胰島組的生存期明顯短于海藻痠鈉微囊化胰島組(分彆為平均57.10d和145.17d)。結果 兩種包膜材料製備的微囊化成豬胰島均可存活于糖尿病大鼠體內併髮揮生物功能，但海藻痠鈉微囊化胰島組較瓊脂糖微囊化胰島組的存活期明顯延長。
목적 통과대동일래원적성저이도채용량충불동적면역격리재료진행미낭화포과，병식입당뇨병대서복강내가이비교，이명학량충방법간적우렬。방법 장동일래원적성저이도분성삼조：미미낭화조、해조산납미낭조화경지당미낭조。24지당뇨병대서수궤분위4조：대조조、미미낭화이도이식조、해조산납미낭화이도이식조화경지당미낭화이도이식조，분별진행복강내이도이식。결과 4조당뇨병대서이도이식전혈당수평무현저차이，이식후1주분별위(22.67±1.15)mmol/L，(18.58±2.62)mmol/L，(12.27±1.36)mmol/L화(12.38±2.68)mmol/L，후2조여전2조상비균유현저차이，차생존기명현장우전2조，단경지당미낭화이도조적생존기명현단우해조산납미낭화이도조(분별위평균57.10d화145.17d)。결과 량충포막재료제비적미낭화성저이도균가존활우당뇨병대서체내병발휘생물공능，단해조산납미낭화이도조교경지당미낭화이도조적존활기명현연장。
Objective To observe and compare the functions of two typesmicroencapsulated adult pig pancreatic islets in vivo.Methods The diabetic rats induced by streptozocin were divided into four groups:control group,non-encapsulated islet transplantation group,encapsulated islet with sodium alginate membrane transplantation group and encapsulated islet with agarose membrane transplantation group.All rats received intraperitoneal of islet transplantation.Results There were no significant difference in mean level of the blood glucose before transplantation among four groups.On the 7th day after transplantation,the mean level of the blood glucose in four groups were (22.67±1.15)mmol/L，(18.58±2.66)mmol/L(12.27±1.36)mmol/L and (12.38±2.68) mmol/L,respectively.There were significant difference between two encapsulated islet groups as compared with the other two groups(P＜0.01).The survival period of two encapsulated islet transplantation groups were more longer than that of the other two group(P＜0.05 and P＜0.01) respectively.The survival period of encapsulated islet with sodium alginate membrane transplantation group was longer than that of encapsulated islet with agarose membrane transplantation group(P＜0.05).Conclusion Microencapsulated adult pig islets with sodium alginatea and agarose can survive in diabetic rats with their biological activity,but the survival period of encapsulated islet with sodium alginate membrane transplantation group was longer than that of encapsulated islet with agarose membrane transplantation group.
