CAJ | 학술논문

目的调查浙江省龙泉市肾综合征出血热(HFRS)疫源地小动物中汉坦病毒(HV)的自然感染情况及其流行的基因型.方法用鼠笼在龙泉市查田镇、小梅镇、小梅镇黄南村捕鼠.捕获的小动物进行分类鉴定并解剖取肺脏,用间接免疫荧光法(IFA)检测肺组织中HV抗原,用RT-PCR对HV抗原阳性的样本扩增部分S片段核苷酸序列并测序,构建系统发生树进行基因分型.结果共捕获小动物319只,其中野外312只,室内7只.黑线姬鼠和东方田鼠为野外优势鼠种.肺组织标本中共检测到9份抗原阳性,带病毒率为4.97%,用部分S片段(620～999nt)的核苷酸序列构建系统发生树,结果表明均为汉滩病毒(HTNV),并与Z251分离株的亲缘关系最近.结论龙泉市野外主要存在以黑线姬鼠为宿主的HTNV感染,且携带率较高.
목적 조사절강성룡천시신종합정출혈열(HFRS)역원지소동물중한탄병독(HV)적자연감염정황급기류행적기인형.방법 용서롱재룡천시사전진、소매진、소매진황남촌포서.포획적소동물진행분류감정병해부취폐장,용간접면역형광법(IFA)검측폐조직중HV항원,용RT-PCR대HV항원양성적양본확증부분S편단핵감산서렬병측서,구건계통발생수진행기인분형.결과 공포획소동물319지,기중야외312지,실내7지.흑선희서화동방전서위야외우세서충.폐조직표본중공검측도9빈항원양성,대병독솔위4.97%,용부분S편단(620～999nt)적핵감산서렬구건계통발생수,결과표명균위한탄병독(HTNV),병여Z251분리주적친연관계최근.결론 룡천시야외주요존재이흑선희서위숙주적HTNV감염,차휴대솔교고.
Objective To investigate the situation of the natural infection of hantaviruses (HV) in small mammals and to provide evidence for the control and prevention of hemorrhagic fever with renal syndrome (HFRS) in Longquan area,Zhejiang province. Methods Small mammals were captured by night trap, and lung tissue samples were collected and stored in liquid nitrogen. HV antigens were detected by indirect immuno-fluorescence assay (IFA). The partial S genome segment sequences were amplified by RT-PCR. DNAStar program was used for editing and comparing the sequences. Phylogeny was analyzed through PAUP*4.0 software. Results 319 small animals were collected in Longquan, and 9 hantavirus antigen-positive samples were identified. The positive rate of hantavirus in Apodemus agrarius was 4.97% . Phylogenetic tree constructed by partial S segment (620-999 nt) showed that the 9 strains carried by A agrarius from Longquan all belonged to HTNV,and had a closer evolutionary relationship with isolate Z251 from Zhejiang province. Conclusion Our results indicated that the main host was A. agrarius and the infection rate of HTNV was high in Longquan area.