国际免疫学杂志
國際免疫學雜誌
국제면역학잡지
INTERNATIONAL JOURNAL OF IMMUNOLOGY
2011年
5期
297-301
,共5页
陈玲%徐雯雯%韩妙君%郭彦%王珏%崔文庆%贾曼红%马艳玲%陆林%张华堂
陳玲%徐雯雯%韓妙君%郭彥%王玨%崔文慶%賈曼紅%馬豔玲%陸林%張華堂
진령%서문문%한묘군%곽언%왕각%최문경%가만홍%마염령%륙림%장화당
HIV-1/AIDS:CD4+T细胞%CD3/CD28抗体%协同刺激%细胞亚群
HIV-1/AIDS:CD4+T細胞%CD3/CD28抗體%協同刺激%細胞亞群
HIV-1/AIDS:CD4+T세포%CD3/CD28항체%협동자격%세포아군
HIV/AIDS%CD4＋ T cells%CD3/CD28 antibody%Costimulation%Cell subsets
目的通过CD3/CD28抗体协同刺激活化原代CD4+T细胞，以复制具有抵抗和清除HIV-1能力的“Levine现象”，建立相关机理研究的实验模式，分析效应细胞的亚群特征。方法从外周血单个核细胞中分选高纯度的CD4+T细胞，以PHA/IL-2为对照，经CD3/CD28抗体刺激培养后，以CD45RO、CD62L和CCR7进行单细胞流式分析，鉴定细胞亚群及CCR5、CXCR4表达水平，以实时荧光定量法测定培养上清中的病毒载量。结果CD3/CD28抗体刺激下CD4+T细胞明显增殖活化，原态细胞减少，明显转为记忆细胞表型，特别是M2亚群和TCM亚群细胞增多，未发现典型的TEMRA亚群。与PHA/IL-2相反，CD3/CD28抗体明显下调CCR5，并使早期感染者的病毒载量始终维持低于检出线水平(＜50 IU/ml)。结论本研究以“Levine现象”为线索，验证和复制了诱导靶细胞抵抗和清除HIV-1感染的研究体系，为进一步探索新的防治HIV/AIDS的细胞分子机理奠定了基础。
目的通過CD3/CD28抗體協同刺激活化原代CD4+T細胞，以複製具有牴抗和清除HIV-1能力的“Levine現象”，建立相關機理研究的實驗模式，分析效應細胞的亞群特徵。方法從外週血單箇覈細胞中分選高純度的CD4+T細胞，以PHA/IL-2為對照，經CD3/CD28抗體刺激培養後，以CD45RO、CD62L和CCR7進行單細胞流式分析，鑒定細胞亞群及CCR5、CXCR4錶達水平，以實時熒光定量法測定培養上清中的病毒載量。結果CD3/CD28抗體刺激下CD4+T細胞明顯增殖活化，原態細胞減少，明顯轉為記憶細胞錶型，特彆是M2亞群和TCM亞群細胞增多，未髮現典型的TEMRA亞群。與PHA/IL-2相反，CD3/CD28抗體明顯下調CCR5，併使早期感染者的病毒載量始終維持低于檢齣線水平(＜50 IU/ml)。結論本研究以“Levine現象”為線索，驗證和複製瞭誘導靶細胞牴抗和清除HIV-1感染的研究體繫，為進一步探索新的防治HIV/AIDS的細胞分子機理奠定瞭基礎。
목적통과CD3/CD28항체협동자격활화원대CD4+T세포，이복제구유저항화청제HIV-1능력적“Levine현상”，건립상관궤리연구적실험모식，분석효응세포적아군특정。방법종외주혈단개핵세포중분선고순도적CD4+T세포，이PHA/IL-2위대조，경CD3/CD28항체자격배양후，이CD45RO、CD62L화CCR7진행단세포류식분석，감정세포아군급CCR5、CXCR4표체수평，이실시형광정량법측정배양상청중적병독재량。결과CD3/CD28항체자격하CD4+T세포명현증식활화，원태세포감소，명현전위기억세포표형，특별시M2아군화TCM아군세포증다，미발현전형적TEMRA아군。여PHA/IL-2상반，CD3/CD28항체명현하조CCR5，병사조기감염자적병독재량시종유지저우검출선수평(＜50 IU/ml)。결론본연구이“Levine현상”위선색，험증화복제료유도파세포저항화청제HIV-1감염적연구체계，위진일보탐색신적방치HIV/AIDS적세포분자궤리전정료기출。
Objective To set up a novel research model by reproducing “Levine phenomenon” which harbours an unusual anti-HIV/AIDS activity. Methods CD4 ＋ T cells were purified from peripheral blood mononuclear cells by negative selection using MACS system. Cells were then stimulated with CD3/CD28 antibodies immobilized on polystyrene beads with PHA/IL-2 as control for 6 days. Cellular phenotypes were analyzed by flow cytometry. Viral load of HIV-infected cells in culture was monitored by Real-time PCR. Results Costimulated cells showed higher expression of activation markers and switched their naive phenotypes to memory predominance with decreased CCR5 expression. With continuous costimulation,in viral production cells from an early infected individual was suppressed to undetectable level. Conclusion The anti-HIV system described by Levine et al can be readily estabhshed de novo in house and serves as a useful model for novel antiviral mechanisms.
