CAJ | 학술논문

目的探讨环氧化酶-2(COX-2)、表皮生长因子受体(EGFR)在星形细胞瘤中的表达及其相关性.方法用免疫组织化学S-P法检测68例不同病理级别星形细胞瘤组织与5例正常脑组织中COX-2、EGFR及PCNA蛋白的表达,并计算增殖指数(PI),分析COX-2表达与EGFR及PI之间的相关性.结果正常脑组织中COX-2、EGFR表达均为阴性,高度恶性组的COX-2阳性表达率明显高于低度恶性组(73.53%vs44.18%,P<0.01),高度恶性组PI(%)明显高于低度恶性组和对照组(46.11±10.68 vs 23.04±6.25,4.52±0.95,P<0.01).在低度恶性组与高度恶性组中,COX-2表达阳性的肿瘤其PI明显高于阴性者(P<0.01).高度恶性组的EGFR阳性表达率明显高于低度恶性组(67.65%vs 38.24%,P<0.01).COX-2蛋白在EGFR阳性组的表达明显高于阴性组.结论 COX-2、EGFR的表达与星形细胞瘤的病理分级有关,COX-2可促进肿瘤细胞增殖,并与EGFR的表达密切相关,EGFR信号通路可能参与COX-2表达的调控.
목적 탐토배양화매-2(COX-2)、표피생장인자수체(EGFR)재성형세포류중적표체급기상관성.방법 용면역조직화학S-P법검측68례불동병리급별성형세포류조직여5례정상뇌조직중COX-2、EGFR급PCNA단백적표체,병계산증식지수(PI),분석COX-2표체여EGFR급PI지간적상관성.결과 정상뇌조직중COX-2、EGFR표체균위음성,고도악성조적COX-2양성표체솔명현고우저도악성조(73.53%vs44.18%,P<0.01),고도악성조PI(%)명현고우저도악성조화대조조(46.11±10.68 vs 23.04±6.25,4.52±0.95,P<0.01).재저도악성조여고도악성조중,COX-2표체양성적종류기PI명현고우음성자(P<0.01).고도악성조적EGFR양성표체솔명현고우저도악성조(67.65%vs 38.24%,P<0.01).COX-2단백재EGFR양성조적표체명현고우음성조.결론 COX-2、EGFR적표체여성형세포류적병리분급유관,COX-2가촉진종류세포증식,병여EGFR적표체밀절상관,EGFR신호통로가능삼여COX-2표체적조공.
Objective To investigate the expression of cyclooxygenase-2 (COX-2) and epidermal growth factor receptor (EGFR) in astrecytomas, as well as the correlation between them. Methods The expression of COX-2, EGFR and PCNA were respectively detected by immunohistochmical (S-P) method in 68 astrocytomas and 5 cases normal brain tissue. Proliferation index (PI) was calculated and the correlation of COX-2, EGFR and PI was analyzed. Results COX-2 and EGFR were negative expression in normal brain tissue. The positive expression rate of COX-2 and EGFR in high grade astrocytomas was significantly higher than that in low grade astrocytomas(73.53% vs 44. 18% ,67.65% vs 38.24%, P <0. 01 ), and the PI was significantly higher than that in low grade astrocytumas as well as normal brain tissue(46.11 ± 10. 68vs 23. 04±6. 25,4. 52±0. 95, P <0. 01 ). The PI in COX-2 positive group was higher than that in negative group( P <0. 01 ). The positive expression rate of COX-2 in the group with EGFR positive expression was higher than that in the negative group. Conclusions The expression of COX-2 and EGFR was related to pathological feature of astrocytomas. COX-2 may promote the proliferation of tumor cells. There was a static correlation between the expression of EGFR and COX-2 in astrocytomas. EGFR signal transduction probably modulated the expression of COX-2 in astrocytomas cells.