CAJ | 학술논문

目的观察德氮吡格(TNBG)对人肝癌细胞QGY-7701亚细胞蛋白表达的影响,探讨其影响脂代谢的分子机制. 方法分别提取TNBG处理前后人肝癌细胞QGY-7701的细胞质、细胞膜和细胞核蛋白进行双向电泳,PDQuest 7.4.0软件分析比对图像,采用基质辅助激光解吸电离飞行时间质谱技术(MALDI-TOF-MS)鉴定差异蛋白质点. 结果 TNBG作用于人肝癌细胞QGY-770172h后,细胞质的差异表达蛋白质点有56个,细胞膜的差异表达蛋白质点有65个,细胞核的差异表达蛋白质点有34个,共计155个.利用MALDI-TOF-MS技术鉴定出其中33个差异表达蛋白质点,其中包括与脂质合成有关的10个蛋白质点和与脂质降解、转运有关的7个蛋白质点,如3-羟基-3甲基戊二酸单酰辅酶A还原酶、角鲨烯合成酶、低密度脂蛋白受体、三磷酸腺柠檬酸裂解酶、甘油醛-3-磷酸脱氢酶、甘油-3-磷酸酰基转移酶、长链酯辅酶A脱氢酶等,这些都是固醇调节元件结合蛋白调控的靶基因.结论 TNBG可能通过固醇调节元件结合蛋白途径增加胆固醇和甘油三酯合成,导致肿瘤细胞内脂滴大量聚积.
목적 관찰덕담필격(TNBG)대인간암세포QGY-7701아세포단백표체적영향,탐토기영향지대사적분자궤제. 방법 분별제취TNBG처리전후인간암세포QGY-7701적세포질、세포막화세포핵단백진행쌍향전영,PDQuest 7.4.0연건분석비대도상,채용기질보조격광해흡전리비행시간질보기술(MALDI-TOF-MS)감정차이단백질점. 결과 TNBG작용우인간암세포QGY-770172h후,세포질적차이표체단백질점유56개,세포막적차이표체단백질점유65개,세포핵적차이표체단백질점유34개,공계155개.이용MALDI-TOF-MS기술감정출기중33개차이표체단백질점,기중포괄여지질합성유관적10개단백질점화여지질강해、전운유관적7개단백질점,여3-간기-3갑기무이산단선보매A환원매、각사희합성매、저밀도지단백수체、삼린산선저몽산렬해매、감유철-3-린산탈경매、감유-3-린산선기전이매、장련지 보매A탈경매등,저사도시고순조절원건결합단백조공적파기인.결론 TNBG가능통과고순조절원건결합단백도경증가담고순화감유삼지합성,도치종류세포내지적대량취적.
Objective To investigate the clinical efficacy ofcryotherapy ablation treatment for advanced hepatocellular carcinoma,and to analyse the predictive factors of cryotherapy ablation treatment.Methods 190patients of hepatitis B-related advanced HCC from 2005 to 2008 in our hospital underwent curative cryoablation.We used clinical cohort method to analyze cryoablation group (147 cases) and control group (43 cases).The median OS (over survival time) and TTP (time to disease progression) were compared.We also evaluated the clinical significance of age,gender,location of portal vein tumor thrombus,HBeAg,tumor histological grade,Child-Pugh classification,end-stage liver disease (MELD) score,advanced liver cancer prediction system (ALCPS) score and the Eastern Cooperative Oncology Group performance status (ECOG PS) score for predicting the efficacy of cryoablation.Two Groups were compared with the x2 test.Survival rates were estimated by the Kaplan-Meier method and compared by the log rank test.The Cox proportional hazards model was used to determine the independent factors on survival based on the variables selected in univariate analysis.Results Median survival time of cryoablation goup and Control group were 7.5 (4.2 to 14.6) months and 3.2 (1.2 to 8.6) months,median TTP were 3.5 (2.5 to 4.5) months and 1.5 (1.0 to 3.5 months),the differences between were statistically significant (P＜0.05).Median OS and TTP of advanced HCC patients who had Well differentiated tumor,Child-pugh A-class and low score of MELD score,ALCPS score; ECOG PS score were significantly longer than that of the poorly differentiated tumor,Child-pugh B-class and the high those scores (P＜ 0.05).ECOG PS (P＜ 0.05,95％ CI 1.074 -2.143) and ALCPS (P＜0.05,95％ CI 1.005-2.121) were independent predictors for OS of advanced HCC.Conclusions Cryoablation treatment can prolong median OS and TTP of advanced HCC.ECOG PS and ALCPS are important predictors for survival time of advanced HCC.