中华劳动卫生职业病杂志
中華勞動衛生職業病雜誌
중화노동위생직업병잡지
CHINESE JOURNAL OF INDUSTRIAL HYGIENE AND OCCUPATIONAL DISEASES
2011年
5期
321-323
,共3页
杨桂丽%杨洁%章军%徐玉英%韦巧慧%孙向珏%顾晓敏%张寅%丁秋萍%郑一凡%杨军%朱心强
楊桂麗%楊潔%章軍%徐玉英%韋巧慧%孫嚮玨%顧曉敏%張寅%丁鞦萍%鄭一凡%楊軍%硃心彊
양계려%양길%장군%서옥영%위교혜%손향각%고효민%장인%정추평%정일범%양군%주심강
纳米颗粒%血小板聚集%凝血酶原时间%凝血酶时间
納米顆粒%血小闆聚集%凝血酶原時間%凝血酶時間
납미과립%혈소판취집%응혈매원시간%응혈매시간
Nanoparticles%Platelet aggregation%Prothrombin time%Thrombin time
目的 观察多壁纳米碳圈(MWCNOs)对大鼠血小板聚集和止血功能的影响.方法 采用Born比浊法通过体内和体外试验测定MWCNOs对大鼠血小板聚集的影响.(1)体外试验:测定溶剂和不同剂量MWCNOs(0.2、2.0、20.0 μg/ml)对大鼠血小板聚集的影响.(2)体内试验:将20只成年健康雄性SD大鼠随机分为溶剂组和MWCNOs组(2、4和8 mg/kg),观察尾静脉注射染毒12 h后MWCNOs对血小板数量、出血时间和凝血指标的影响.同时观察尾静脉注射4 mg/kg MWCNOs和等体积溶剂作用不同时间(0.5、6和12 h)后对血小板数量和血小板聚集的影响.结果 MWCNOs对由二磷酸腺苷引起的血小板聚集有明显的抑制作用,并且随着剂量的增加,抑制作用增强.最高剂量组(20.0 μg/ml)血小板聚集率(50.0%±6.9%)明显低于溶剂组(73.2%±4.3%),差异有统计学意义(P<0.01).体内试验结果同样观察到MWCNOs存在抑制血小板聚集的作用,2、4、8 mg/kg MWCNOs组的血小板聚集率(分别为51.2%±4.5%、54.3%±7.8%、56.8%±3.0%)均低于溶剂组(64.3%±3.4%),但差异无统计学意义(P>0.05).未发现MWCNOs对大鼠血小板数量、出血时间和凝血指标有明显影响.结论 在该研究条件下,MWCNOs对血小板聚集可能存在抑制作用,但并不影响机体的止血功能.
目的 觀察多壁納米碳圈(MWCNOs)對大鼠血小闆聚集和止血功能的影響.方法 採用Born比濁法通過體內和體外試驗測定MWCNOs對大鼠血小闆聚集的影響.(1)體外試驗:測定溶劑和不同劑量MWCNOs(0.2、2.0、20.0 μg/ml)對大鼠血小闆聚集的影響.(2)體內試驗:將20隻成年健康雄性SD大鼠隨機分為溶劑組和MWCNOs組(2、4和8 mg/kg),觀察尾靜脈註射染毒12 h後MWCNOs對血小闆數量、齣血時間和凝血指標的影響.同時觀察尾靜脈註射4 mg/kg MWCNOs和等體積溶劑作用不同時間(0.5、6和12 h)後對血小闆數量和血小闆聚集的影響.結果 MWCNOs對由二燐痠腺苷引起的血小闆聚集有明顯的抑製作用,併且隨著劑量的增加,抑製作用增彊.最高劑量組(20.0 μg/ml)血小闆聚集率(50.0%±6.9%)明顯低于溶劑組(73.2%±4.3%),差異有統計學意義(P<0.01).體內試驗結果同樣觀察到MWCNOs存在抑製血小闆聚集的作用,2、4、8 mg/kg MWCNOs組的血小闆聚集率(分彆為51.2%±4.5%、54.3%±7.8%、56.8%±3.0%)均低于溶劑組(64.3%±3.4%),但差異無統計學意義(P>0.05).未髮現MWCNOs對大鼠血小闆數量、齣血時間和凝血指標有明顯影響.結論 在該研究條件下,MWCNOs對血小闆聚集可能存在抑製作用,但併不影響機體的止血功能.
목적 관찰다벽납미탄권(MWCNOs)대대서혈소판취집화지혈공능적영향.방법 채용Born비탁법통과체내화체외시험측정MWCNOs대대서혈소판취집적영향.(1)체외시험:측정용제화불동제량MWCNOs(0.2、2.0、20.0 μg/ml)대대서혈소판취집적영향.(2)체내시험:장20지성년건강웅성SD대서수궤분위용제조화MWCNOs조(2、4화8 mg/kg),관찰미정맥주사염독12 h후MWCNOs대혈소판수량、출혈시간화응혈지표적영향.동시관찰미정맥주사4 mg/kg MWCNOs화등체적용제작용불동시간(0.5、6화12 h)후대혈소판수량화혈소판취집적영향.결과 MWCNOs대유이린산선감인기적혈소판취집유명현적억제작용,병차수착제량적증가,억제작용증강.최고제량조(20.0 μg/ml)혈소판취집솔(50.0%±6.9%)명현저우용제조(73.2%±4.3%),차이유통계학의의(P<0.01).체내시험결과동양관찰도MWCNOs존재억제혈소판취집적작용,2、4、8 mg/kg MWCNOs조적혈소판취집솔(분별위51.2%±4.5%、54.3%±7.8%、56.8%±3.0%)균저우용제조(64.3%±3.4%),단차이무통계학의의(P>0.05).미발현MWCNOs대대서혈소판수량、출혈시간화응혈지표유명현영향.결론 재해연구조건하,MWCNOs대혈소판취집가능존재억제작용,단병불영향궤체적지혈공능.
Objective To observe the effects of multiwall carbon nano-onions (MWCNOs) on platelet aggregation and hemostatic function. Methods The platelet aggregation was determined with Born' s method at different concentration of MWCNOs (0, 0.2, 2.0, 20.0 μg/ml) in vitro. Twenty male SD rats were randomly divided into 4 groups which were exposed to 0, 2, 4 and 8 mg/kg MWCNOs, respectively. Then platelet count, platelet aggregation, activated partial thromboplastin time (APTT), prothrombin time (PT), thrombin time (TT), bleeding time (BT) and platelet count (PC) were measured at 12 h after receiving tail intravenous injection of MWCNOs. The effects of MWCNOs (4 mg/kg) on platelet aggregation and platelet count at different time points were observed. Results In vitro, MWCNOs exhibited the potent inhibitory effects on rat platelet aggregation caused by ADP in a concentration-dependent manner. The platelet aggregation in the highest dosage of 20.0 μg/ml group was 50.0%±6.9% which was significantly lower than that ( 73.2% ±4.3% ) in control group (P<0.01). In vivo, the highest inhibitory was up to 20.4% , but there was no significant difference, as compared with control group. MWCNOs did not affect the APTT, PT, TT, BT and PC. Conclusion Under this experimental condition, MWCNOs might inhibit platelet aggregation but not affect hemostatic function.
