中华糖尿病杂志
中華糖尿病雜誌
중화당뇨병잡지
CHINES JOURNAL OF DLABETES MELLITUS
2011年
3期
252-255
,共4页
周晰溪%李会芳%王玉明%吴于滨%宋滇平
週晰溪%李會芳%王玉明%吳于濱%宋滇平
주석계%리회방%왕옥명%오우빈%송전평
糖尿病,2型%糖尿病肾病%基因%环氧化酶2
糖尿病,2型%糖尿病腎病%基因%環氧化酶2
당뇨병,2형%당뇨병신병%기인%배양화매2
Diabetes mellitus,type 2%Diabetic nephropathy%Genes%Cyclooxygenase-2
目的 探讨环氧化酶2基因启动子区-765G/C多态性与昆明地区汉族2型糖尿病患者糖尿病肾病发生、发展的相关性.方法 选取2008年1月至12月昆明医学院第一附属医院糖尿病科收治住院的2型糖尿病患者252例,根据24 h尿白蛋白排泄率或随机尿白蛋白/肌酐比值分至无糖尿病肾病组(n=100)、隐性糖尿病肾病组(n=78)、显性糖尿病肾病组(n=74).运用聚合酶链反应-限制性片段长度多态性方法检测环氧化酶2基因启动子区-765G/C多态性.检测血糖、血脂和其他生化指标.运用χ2检验比较各组基因型频率和等位基因频率,运用方差分析比较各组相关临床及生化指标,采用logistic回归分析评价影响糖尿病肾病发生、发展的危险因素.结果 两糖尿病肾病组-765GG基因型频率与无糖尿病肾病组存在明显差异(0.901 vs 0.810;χ2=4.309,P＜0.05＝.糖尿病肾病患者中,-765GG基因型携带者空腹血糖、餐后2 h血糖与非携带者存在明显差异[分别为(7.9±4.0)vs(5.9±1.7)mmol/L,(12±5)vs(9±4)mmol/L;t值分别为0.001、0.020,均P＜0.05＝.logistic回归分析提示,-765GG基因型是糖尿病肾病发生的独立危险因素之一[比值比(OR)=3.25,95%可信区间(CI)为1.336～7.901].结论 在昆明地区汉族2型糖尿病患者中,环氧化酶2基因启动子区-765GG基因型与糖尿病肾病的发生、发展有关.
目的 探討環氧化酶2基因啟動子區-765G/C多態性與昆明地區漢族2型糖尿病患者糖尿病腎病髮生、髮展的相關性.方法 選取2008年1月至12月昆明醫學院第一附屬醫院糖尿病科收治住院的2型糖尿病患者252例,根據24 h尿白蛋白排洩率或隨機尿白蛋白/肌酐比值分至無糖尿病腎病組(n=100)、隱性糖尿病腎病組(n=78)、顯性糖尿病腎病組(n=74).運用聚閤酶鏈反應-限製性片段長度多態性方法檢測環氧化酶2基因啟動子區-765G/C多態性.檢測血糖、血脂和其他生化指標.運用χ2檢驗比較各組基因型頻率和等位基因頻率,運用方差分析比較各組相關臨床及生化指標,採用logistic迴歸分析評價影響糖尿病腎病髮生、髮展的危險因素.結果 兩糖尿病腎病組-765GG基因型頻率與無糖尿病腎病組存在明顯差異(0.901 vs 0.810;χ2=4.309,P＜0.05＝.糖尿病腎病患者中,-765GG基因型攜帶者空腹血糖、餐後2 h血糖與非攜帶者存在明顯差異[分彆為(7.9±4.0)vs(5.9±1.7)mmol/L,(12±5)vs(9±4)mmol/L;t值分彆為0.001、0.020,均P＜0.05＝.logistic迴歸分析提示,-765GG基因型是糖尿病腎病髮生的獨立危險因素之一[比值比(OR)=3.25,95%可信區間(CI)為1.336～7.901].結論 在昆明地區漢族2型糖尿病患者中,環氧化酶2基因啟動子區-765GG基因型與糖尿病腎病的髮生、髮展有關.
목적 탐토배양화매2기인계동자구-765G/C다태성여곤명지구한족2형당뇨병환자당뇨병신병발생、발전적상관성.방법 선취2008년1월지12월곤명의학원제일부속의원당뇨병과수치주원적2형당뇨병환자252례,근거24 h뇨백단백배설솔혹수궤뇨백단백/기항비치분지무당뇨병신병조(n=100)、은성당뇨병신병조(n=78)、현성당뇨병신병조(n=74).운용취합매련반응-한제성편단장도다태성방법검측배양화매2기인계동자구-765G/C다태성.검측혈당、혈지화기타생화지표.운용χ2검험비교각조기인형빈솔화등위기인빈솔,운용방차분석비교각조상관림상급생화지표,채용logistic회귀분석평개영향당뇨병신병발생、발전적위험인소.결과 량당뇨병신병조-765GG기인형빈솔여무당뇨병신병조존재명현차이(0.901 vs 0.810;χ2=4.309,P＜0.05＝.당뇨병신병환자중,-765GG기인형휴대자공복혈당、찬후2 h혈당여비휴대자존재명현차이[분별위(7.9±4.0)vs(5.9±1.7)mmol/L,(12±5)vs(9±4)mmol/L;t치분별위0.001、0.020,균P＜0.05＝.logistic회귀분석제시,-765GG기인형시당뇨병신병발생적독립위험인소지일[비치비(OR)=3.25,95%가신구간(CI)위1.336～7.901].결론 재곤명지구한족2형당뇨병환자중,배양화매2기인계동자구-765GG기인형여당뇨병신병적발생、발전유관.
Objective To determine the possible effect of cyclooxygenase-2 (COX-2) -765G/C polymorphism on the risk of diabetic nephropathy (DN) in patients with type 2 diabetes mellitus (T2DM) in Kunming. Methods A total of 252 T2DM patients from the First Affiliated Hospital of Kunming Medical University between January and December 2008 were assigned to the free-DN group (n=100), microalbuminuria DN group (n=78) and macroalbuminuria DN group (n=74) according to 24-hour urinary albumin excretion ratio(24-h UAER) or spot urinary albumin/creatinine (UACR) ratio. The polymorphism of COX-2 gene -765G/C was detected by PCR-RFLP. The genotypes and allele frequency distributions were compared by using χ2 test. The related characteristics of investigated objects were compared by analysis of variance. Logistic regression analysis was performed to calculate correlated markers of DN risk. Results In T2DM patients, -765GG genotype was significantly different between microalbuminuria DN﹢macroalbuminuria DN group and free-DN group (0.901 vs 0.810; χ2=4.309,P<0.05). In DN patients, the levels of fasting plasma glucose (FPG) and 2-hour plasma glucose (2-h PG) of those with -765GG genotype were higher than those without ((7.9±4.0) vs (5.9±1.7) mmol/L, (12±5) vs (9±4) mmol/L, respectively; t values were 0.001 and 0.020, both P<0.05). Logistic regression analysis indicated that -765GG genotype (odds ratio (OR)=3.25; 95%confidence interval (CI): 1.336 to 7.901) was risk factor of DN. Conclusion The COX-2 gene promoter -765GG may be associated with DN in Kunming Han individuals with T2DM.
