乙型肝炎病毒外膜蛋白和核壳蛋白双表达质粒的构建和表达
을형간염병독외막단백화핵각단백쌍표체질립적구건화표체
Construction and expression of hepatitis B virus envelope protein combined with core protein with two multiple cloning sites vector
  • 万方数据
    中华实验和临床病毒学杂志 중화실험화림상병독학잡지
    CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY
    2009年 4期 313-315 ,共3页

    沈宏辉%刘素君%郭雷%侯俊%王志杰%辛绍杰%白冰珂%胡燕%于湘晖%孔维%貌盼勇

    침굉휘%류소군%곽뢰%후준%왕지걸%신소걸%백빙가%호연%우상휘%공유%모반용

    肝炎病毒%乙型%表达的序列标记%病毒包膜蛋白质类%核蛋白质类 肝炎病毒%乙型%錶達的序列標記%病毒包膜蛋白質類%覈蛋白質類
    간염병독%을형%표체적서렬표기%병독포막단백질류%핵단백질류
    Hepatitis B virus%Expressed sequence tags%Viral envelope protcins%Nucleoproteins
    目的 构建同时表达乙型肝炎病毒外膜蛋白和核心蛋白的真核表达质粒并研究其表达效果.方法 利用DNA重组技术,构建由两个CMV启动子分别表达乙型肝炎病毒外膜蛋白和核心蛋白的双表达真核质粒VR-SC,体外真核表达后ELISA检测HBsAg和HBeAg.结果 在乙肝病毒外膜蛋白表达质粒上插入多克隆位点,并插入乙肝病毒核心蛋白的完整表达调控单元,体外真核表达后成功检测到HBsAg和HBeAg.结论 成功构建双表达质粒vR-sc,其表达效果良好,为进一步改造成HBV DNA疫苗打下了基础.
    목적 구건동시표체을형간염병독외막단백화핵심단백적진핵표체질립병연구기표체효과.방법 이용DNA중조기술,구건유량개CMV계동자분별표체을형간염병독외막단백화핵심단백적쌍표체진핵질립VR-SC,체외진핵표체후ELISA검측HBsAg화HBeAg.결과 재을간병독외막단백표체질립상삽입다극륭위점,병삽입을간병독핵심단백적완정표체조공단원,체외진핵표체후성공검측도HBsAg화HBeAg.결론 성공구건쌍표체질립vR-sc,기표체효과량호,위진일보개조성HBV DNA역묘타하료기출.
    Objective To develop a eoexpression plasmid which expressing envelope protein and nucleoprotein of hepatitis B virus and know of its expressing efficiency. Methods The plasmid coexpressing envelope protein and nucleoprotein of hepatitis B virus under the CMV promoter respectively was constructed by gene recombination. Cellular expression was assessed by ELISA. Results Multiple cloning site was inserted into expression vector contain hepatitis B virus PreS2-S gene. And expression unit containing hepatitis B virus PreC-C was cloned into it. HBsAg and HBeAg was detected both in the culture supernatant and in the cells. Conclusion The coexpressing plasmid was constructed successfully and it can express effectively in vitro. This has provided a basis for further research of the therapeutic HBV DNA vaccine.
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