中华肿瘤杂志
中華腫瘤雜誌
중화종류잡지
CHINESE JOURNAL OF ONCOLOGY
2011年
3期
197-201
,共5页
刘勇%张欣%邱元正%黄东海%周小娟%谭平清%蔡耿明%戴耀章%余长云%肖健云%田勇泉
劉勇%張訢%邱元正%黃東海%週小娟%譚平清%蔡耿明%戴耀章%餘長雲%肖健雲%田勇泉
류용%장흔%구원정%황동해%주소연%담평청%채경명%대요장%여장운%초건운%전용천
喉肿瘤%肿瘤转移%复发%预后%EphA2
喉腫瘤%腫瘤轉移%複髮%預後%EphA2
후종류%종류전이%복발%예후%EphA2
Laryngeal neoplasms%Neoplasm metastasis%Recurrence%Prognosis%EphA2
目的 检测促红细胞生成素产生肝细胞受体A2(EphA2)蛋白在喉鳞癌组织及细胞系中的表达,探讨其表达与喉鳞癌临床病理特征和预后之间的关系.方法 采用Western blot技术,检测EphA2蛋白在喉鳞癌细胞株Hep-2及头颈部永生化上皮细胞株NP-69中的表达;采用免疫组织化学技术,检测EphA2蛋白在88例喉鳞癌及16例癌旁黏膜组织中的表达,并分析EphA2与临床病理特征和预后的关系.结果 EphA2蛋白在Hep-2细胞株中的表达高于NP-69细胞株.EphA2蛋白在喉鳞癌组织及癌旁黏膜组织中的刚性表达率分别为80.7%和43.8%,差异有统计学意义(P＜0.001).EphA2高表达与喉鳞癌的临床分期(P=0.005)、淋巴结转移(P=0.025)和复发(P=0.021)密切相关.Kaplan-Meier生存分析结果显示,EphA2高表达组与低表达组的5年无瘤生存率分别为33.3%和63.2%,5年生存率分别为46.7%和81.6%,差异有统计学意义(P=0.003,P=0.002).EphA2表达水平结合临床分期对喉鳞癌预后具有更好的预测价值.单因素及多因素Cox比例风险回归模型进一步显示,EphA2表达水平为喉鳞癌预后的独立影响因素(P=0.019).结论 EphA2蛋白在喉鳞癌组织和细胞中的表达显著升高,且其高表达与喉鳞癌患者的复发、转移及预后密切相关.EphA2可能在喉鳞癌的发生、发展中有重要作用,有望成为评估喉鳞癌复发、转移及预后的重要分子标志物.
目的 檢測促紅細胞生成素產生肝細胞受體A2(EphA2)蛋白在喉鱗癌組織及細胞繫中的錶達,探討其錶達與喉鱗癌臨床病理特徵和預後之間的關繫.方法 採用Western blot技術,檢測EphA2蛋白在喉鱗癌細胞株Hep-2及頭頸部永生化上皮細胞株NP-69中的錶達;採用免疫組織化學技術,檢測EphA2蛋白在88例喉鱗癌及16例癌徬黏膜組織中的錶達,併分析EphA2與臨床病理特徵和預後的關繫.結果 EphA2蛋白在Hep-2細胞株中的錶達高于NP-69細胞株.EphA2蛋白在喉鱗癌組織及癌徬黏膜組織中的剛性錶達率分彆為80.7%和43.8%,差異有統計學意義(P＜0.001).EphA2高錶達與喉鱗癌的臨床分期(P=0.005)、淋巴結轉移(P=0.025)和複髮(P=0.021)密切相關.Kaplan-Meier生存分析結果顯示,EphA2高錶達組與低錶達組的5年無瘤生存率分彆為33.3%和63.2%,5年生存率分彆為46.7%和81.6%,差異有統計學意義(P=0.003,P=0.002).EphA2錶達水平結閤臨床分期對喉鱗癌預後具有更好的預測價值.單因素及多因素Cox比例風險迴歸模型進一步顯示,EphA2錶達水平為喉鱗癌預後的獨立影響因素(P=0.019).結論 EphA2蛋白在喉鱗癌組織和細胞中的錶達顯著升高,且其高錶達與喉鱗癌患者的複髮、轉移及預後密切相關.EphA2可能在喉鱗癌的髮生、髮展中有重要作用,有望成為評估喉鱗癌複髮、轉移及預後的重要分子標誌物.
목적 검측촉홍세포생성소산생간세포수체A2(EphA2)단백재후린암조직급세포계중적표체,탐토기표체여후린암림상병리특정화예후지간적관계.방법 채용Western blot기술,검측EphA2단백재후린암세포주Hep-2급두경부영생화상피세포주NP-69중적표체;채용면역조직화학기술,검측EphA2단백재88례후린암급16례암방점막조직중적표체,병분석EphA2여림상병리특정화예후적관계.결과 EphA2단백재Hep-2세포주중적표체고우NP-69세포주.EphA2단백재후린암조직급암방점막조직중적강성표체솔분별위80.7%화43.8%,차이유통계학의의(P＜0.001).EphA2고표체여후린암적림상분기(P=0.005)、림파결전이(P=0.025)화복발(P=0.021)밀절상관.Kaplan-Meier생존분석결과현시,EphA2고표체조여저표체조적5년무류생존솔분별위33.3%화63.2%,5년생존솔분별위46.7%화81.6%,차이유통계학의의(P=0.003,P=0.002).EphA2표체수평결합림상분기대후린암예후구유경호적예측개치.단인소급다인소Cox비례풍험회귀모형진일보현시,EphA2표체수평위후린암예후적독립영향인소(P=0.019).결론 EphA2단백재후린암조직화세포중적표체현저승고,차기고표체여후린암환자적복발、전이급예후밀절상관.EphA2가능재후린암적발생、발전중유중요작용,유망성위평고후린암복발、전이급예후적중요분자표지물.
Objective To evaluate the expression of EphA2 protein in tissue specimens and cell lines of laryngeal squamous cell carcinoma ( LSCC), and to further study the correlation of EphA2 protein expression with clinicopathological characteristics and prognosis in LSCC. Methods Western blot was applied to assess the EphA2 protein expression in LSCC cell line Hep-2 cells and the head and neck immortalized epithelial cell line NP-69 cells. Immunohistochemical staining was performed on paraffin sections of 88 cases of LSCC specimens and 16 cases of adjcent normal tissue samples to investigate the EphA2 protein expression, and to futher elucidate its correlation with clinicopathological characteristics.Results Compared with the NP-69 cells, EphA2 expression in LSCC cell line Hep-2 cells was upregulated.The positive rates of EphA2 expression in LSCC and adjcent normal tissues samples were 80. 7% and 43.8%,respectively, with a significant difference between the two groups ( P ＜ 0. 001 ). EphA2 overexpresion was closely correlated with clinical stage ( Ⅰ + Ⅱ / Ⅲ + Ⅳ, P = 0. 005 ), metastasis ( P = 0. 025 ) and recurrence(P = 0. 021 ) in LSCC. Furthermore, patients with EphA2 overexpression had poorer tumor-free survival and 5-year overall survival compared with that in patients with low EphA2 expression (33.3% vs.63.2%, P =0.003; 46.7% vs. 81.6%, P = 0. 002 ). EphA2 expression combined with clinical stage provided a better predictive value in prognosis. Univariate and multivariate Cox regression analysis revealed that EphA2 expression is an independent prognostic factor for patients with LSCC( P =0.019). Conclusions The results of this study demonstrate that EphA2 protein expression is significantly increased in LSCC tissues and cell lines, and EphA2 protein overexpression is associated with tumor recurrence, metastasis and poorer prognosis in LSCC patients. These results suggest that EphA2 may play a critical role in the initiation and progression of LSCC, implicating EphA2 as a valuable marker for the prediction of recurrence, metastasis and prognosis in LSCC.
