中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2012年
15期
1058-1061
,共4页
脊髓损伤%神经源性膀胱%神经肽%免疫组织化学染色
脊髓損傷%神經源性膀胱%神經肽%免疫組織化學染色
척수손상%신경원성방광%신경태%면역조직화학염색
Spinal cord injury%Neurogenic bladder%Neuropeptides%Immunohistochemistry staining
目的 观察SD大鼠脊髓损伤痉挛性膀胱形成后膀胱逼尿肌内神经肽Y(NPY)、P物质(SP)和血管活性肠肽(VIP)表达的改变,探讨其与脊髓损伤痉挛性膀胱的关系.方法 成年雄性SD大鼠20只,按随机数字表法分为脊髓损伤组(n=10)和对照组(n=10),脊髓损伤组采用重物坠落法制作大鼠胸段脊髓挫伤模型,对照组只打开椎板不造成脊髓损伤,术后1周所有大鼠行膀胱压力容量测定,确定痉挛性膀胱形成.取膀胱逼尿肌组织,分别行神经纤维银染和NPY、SP、VIP免疫组织化学染色,用测量累积吸光度(IA)的半定量方法评估脊髓损伤及对照组大鼠膀胱逼尿肌内神经肽表达的改变情况.结果 脊髓损伤组大鼠造模术后1周平均膀胱最大容量为(0.71 ±0.24) ml,较正常对照组(1.99±0.42) ml明显降低；平均最大逼尿肌压力值为(32.3±3.1)cm H2O(1 cm H2O =0.098kPa),较正常对照组(21.0±3.0)cnm H2O明显增高；平均膀胱顺应性为(0.020 ±0.009) ml/cm H2O,较正常对照组(0.090±0.020) ml/cm H2O明显降低；上述差异均具有统计学意义(均P＜0.01).脊髓损伤痉挛性膀胱逼尿肌内神经纤维平均含量值为2.58±0.13,较正常对照组值(5.65±0.26)明显减少；NPY平均IA值为3.2±0.5,较正常对照组值(7.3±0.7)明显减低；SP平均IA值为1.7±0.4,较正常对照组值(5.0±0.4)明显减低；VIP平均IA值为2.1±0.4,较正常对照组值(5.5±0.5)明显减低；上述差异均具有统计学意义(均P ＜0.01).结论 大鼠脊髓损伤痉挛性膀胱形成后膀胱逼尿肌内神经肽物质含量明显减少,可能与脊髓损伤后神经源性膀胱形成有关.
目的 觀察SD大鼠脊髓損傷痙攣性膀胱形成後膀胱逼尿肌內神經肽Y(NPY)、P物質(SP)和血管活性腸肽(VIP)錶達的改變,探討其與脊髓損傷痙攣性膀胱的關繫.方法 成年雄性SD大鼠20隻,按隨機數字錶法分為脊髓損傷組(n=10)和對照組(n=10),脊髓損傷組採用重物墜落法製作大鼠胸段脊髓挫傷模型,對照組隻打開椎闆不造成脊髓損傷,術後1週所有大鼠行膀胱壓力容量測定,確定痙攣性膀胱形成.取膀胱逼尿肌組織,分彆行神經纖維銀染和NPY、SP、VIP免疫組織化學染色,用測量纍積吸光度(IA)的半定量方法評估脊髓損傷及對照組大鼠膀胱逼尿肌內神經肽錶達的改變情況.結果 脊髓損傷組大鼠造模術後1週平均膀胱最大容量為(0.71 ±0.24) ml,較正常對照組(1.99±0.42) ml明顯降低；平均最大逼尿肌壓力值為(32.3±3.1)cm H2O(1 cm H2O =0.098kPa),較正常對照組(21.0±3.0)cnm H2O明顯增高；平均膀胱順應性為(0.020 ±0.009) ml/cm H2O,較正常對照組(0.090±0.020) ml/cm H2O明顯降低；上述差異均具有統計學意義(均P＜0.01).脊髓損傷痙攣性膀胱逼尿肌內神經纖維平均含量值為2.58±0.13,較正常對照組值(5.65±0.26)明顯減少；NPY平均IA值為3.2±0.5,較正常對照組值(7.3±0.7)明顯減低；SP平均IA值為1.7±0.4,較正常對照組值(5.0±0.4)明顯減低；VIP平均IA值為2.1±0.4,較正常對照組值(5.5±0.5)明顯減低；上述差異均具有統計學意義(均P ＜0.01).結論 大鼠脊髓損傷痙攣性膀胱形成後膀胱逼尿肌內神經肽物質含量明顯減少,可能與脊髓損傷後神經源性膀胱形成有關.
목적 관찰SD대서척수손상경련성방광형성후방광핍뇨기내신경태Y(NPY)、P물질(SP)화혈관활성장태(VIP)표체적개변,탐토기여척수손상경련성방광적관계.방법 성년웅성SD대서20지,안수궤수자표법분위척수손상조(n=10)화대조조(n=10),척수손상조채용중물추락법제작대서흉단척수좌상모형,대조조지타개추판불조성척수손상,술후1주소유대서행방광압력용량측정,학정경련성방광형성.취방광핍뇨기조직,분별행신경섬유은염화NPY、SP、VIP면역조직화학염색,용측량루적흡광도(IA)적반정량방법평고척수손상급대조조대서방광핍뇨기내신경태표체적개변정황.결과 척수손상조대서조모술후1주평균방광최대용량위(0.71 ±0.24) ml,교정상대조조(1.99±0.42) ml명현강저；평균최대핍뇨기압력치위(32.3±3.1)cm H2O(1 cm H2O =0.098kPa),교정상대조조(21.0±3.0)cnm H2O명현증고；평균방광순응성위(0.020 ±0.009) ml/cm H2O,교정상대조조(0.090±0.020) ml/cm H2O명현강저；상술차이균구유통계학의의(균P＜0.01).척수손상경련성방광핍뇨기내신경섬유평균함량치위2.58±0.13,교정상대조조치(5.65±0.26)명현감소；NPY평균IA치위3.2±0.5,교정상대조조치(7.3±0.7)명현감저；SP평균IA치위1.7±0.4,교정상대조조치(5.0±0.4)명현감저；VIP평균IA치위2.1±0.4,교정상대조조치(5.5±0.5)명현감저；상술차이균구유통계학의의(균P ＜0.01).결론 대서척수손상경련성방광형성후방광핍뇨기내신경태물질함량명현감소,가능여척수손상후신경원성방광형성유관.
Objective To observe the altered expressions of neuropeptide Y,substance P and vasoactive intestinal peptide in detrusor of SD rats after spinal cord injury and explore the relationship of the above neuropeptides and neurogcnic bladder after spinc cord injury.Methods Twenty male clean-grade SD rats,aged 6 weeks,were selected and randomized into spinal cord injury group (n =10) and control group (n =10).Rats in spinal cord injury group were smashed at T10 to cause spinal cord incomplete injury model by the weight drop method while laminectomy alone without smashing was administered in control group.At Week 1 post-operation,all rats were assessed by the maximum bladder capacity,bladder compliance and detrusor pressure for the confirmation of spastic bladder.And all detrusor specimens were marked with argentation and immunohistochemistry for the analyses of nerve fibers,neuropeptide Y,substance P and vasoactive intestinal peptide.The results were evaluated with semiquantitative method to observe the contents of nerve fiber and neuropeptides.Results At Week 1 post-operation, the mean maximum bladder compacity,mean maximum detrusor pressure and mean compliance in SCI rats was 0.71 ± 0.24 ml,32.27 ±3.12 cm H2O and 0.020 ±0.009 ml/cm H2O versus 2.0 ± 0.4 ml,21.0 ± 3.0 cm H2O and 0.090 ± 0.020 ml/cm H2O in normal control group respectively.And the differences were statistically significant (P＜0.01).Meanwhile,the mean content of nerve fibers of neurogenic bladder decreased markedly than that of normal control group ( 2.58 ± 0.1 3 vs 5.65 ± 0.26 ).As compared with the normal control group,the expressions of neuropeptide Y,substance P and vasoactive intestinal peptide (mean integrated optical density:3.2 ± 0.5,1.7 ± 0.4 and 2.1 ± 0.4 respectively) decreased dramatically in SCI rats.And the differences were statistically significant ( P ＜ 0.01 ),Conclusion The number of nerve fibers and the content of neuropeptides significantly decrease in neurogenic bladder after spinal cord injury in rats.The reduction of neuropeptides may be correlated with the formation of neurogenic bladder after spinal cord injury.
