CAJ | 학술논문

通过RT-PCR技术分离了编码小麦线粒体蛋白转运酶TOM7亚基(Translocase of Outer Mitochondrial Membrane subunit 7)的cDNA,并克隆了该基因编码区的基因组DNA,将该基因命名为TaTOM7.TaTOM7是一个没有内含子的基因,其编码的多肽具有一个位于中部的疏水跨膜区和两个分别位于N-端和C-端的亲水区域.来源于真菌、动物和植物的TOM7类似蛋白在疏水跨膜区高度保守,而在亲水区变异很大.在系统发育树中,TOM7类似蛋白可以按动物、植物和真菌分为3个类群.小麦TaTOM7在基因组中以寡拷贝形式存在,它的表达在Ms2近等基因系之间和不同组织之间存在差异.中国春缺体-四体及端二体系分析表明,TaTOM7位于小麦第3染色体同源群.
통과RT-PCR기술분리료편마소맥선립체단백전운매TOM7아기(Translocase of Outer Mitochondrial Membrane subunit 7)적cDNA,병극륭료해기인편마구적기인조DNA,장해기인명명위TaTOM7.TaTOM7시일개몰유내함자적기인,기편마적다태구유일개위우중부적소수과막구화량개분별위우N-단화C-단적친수구역.래원우진균、동물화식물적TOM7유사단백재소수과막구고도보수,이재친수구변이흔대.재계통발육수중,TOM7유사단백가이안동물、식물화진균분위3개류군.소맥TaTOM7재기인조중이과고패형식존재,타적표체재Ms2근등기인계지간화불동조직지간존재차이.중국춘결체-사체급단이체계분석표명,TaTOM7위우소맥제3염색체동원군.
The cDNA encoding TOM7 (translocase of outer mitochondrial membrane subunit 7) like protein in wheat was cloned through RT-PCR,and its genomic DNA fragment was subsequently cloned. This gene was tentatively designated as TaTOM7. It has no intron in the coding region and its product possesses one hydrophobic trans-membrane domain in the middle,and one hydrophilic domain in the N-terminal and C-terminal domain, respectively. The amino acid composition in the trans-membrane domain of the TaTOM7 subunit is highly conserved among plants, animals and fungi. According to the phylogenetic tree,TOM7-like proteins from different species can be classified into three groups, representing plants,animals and fungi respectively. TaTOM7 is a single- or oligo-copy gene in the wheat genome,displaying different expression levels between Ms2 near-isogenic lines in some tissues,suggesting a role of Ms2 in its expression. TaTOM7 was mapped to chromosome group three of wheat.