CAJ | 학술논문

目的:探讨野生型星形胶质细胞(ASC)和运动神经元VSC4.1(VSC)共培养时两者产生活性氧(ROS)的相互影响.方法: MTT法测定比较ASC和VSC在单独培养和共培养时受到兴奋性刺激后生长抑制率;ASC与Hoechst 33342标记的VSC共培养或分别单独培养时,激光共聚焦显微镜实时观察2种情况下两者产生ROS的能力.结果: 较高浓度谷氨酸刺激可使共培养的混合细胞抑制率(IRVSC+ASC)明显高于星形胶质细胞抑制率(IRASC),而较低浓度时IRVSC+ASC高于IRVSC.激光共聚焦显微镜实时观察发现共培养时VSC产生ROS水平明显低于单独培养的VSC,且前者在15 min时出现明显升高,而单独培养的VSC产生的ROS在5 min内达到峰值后即逐渐降低;共培养时ASC产生的ROS在10 min时出现明显升高.结论: ASC和VSC共培养时ASC可降低VSC静息时的ROS水平,在受到兴奋性刺激后ASC延长VSC产生高水平ROS的时程.
목적:탐토야생형성형효질세포(ASC)화운동신경원VSC4.1(VSC)공배양시량자산생활성양(ROS)적상호영향.방법: MTT법측정비교ASC화VSC재단독배양화공배양시수도흥강성자격후생장억제솔;ASC여Hoechst 33342표기적VSC공배양혹분별단독배양시,격광공취초현미경실시관찰2충정황하량자산생ROS적능력.결과: 교고농도곡안산자격가사공배양적혼합세포억제솔(IRVSC+ASC)명현고우성형효질세포억제솔(IRASC),이교저농도시IRVSC+ASC고우IRVSC.격광공취초현미경실시관찰발현공배양시VSC산생ROS수평명현저우단독배양적VSC,차전자재15 min시출현명현승고,이단독배양적VSC산생적ROS재5 min내체도봉치후즉축점강저;공배양시ASC산생적ROS재10 min시출현명현승고.결론: ASC화VSC공배양시ASC가강저VSC정식시적ROS수평,재수도흥강성자격후ASC연장VSC산생고수평ROS적시정.
AIM: To explore the mutual effect of co-culture of wild-type astrocytes (ASC) and motor neurons VSC4.1 (VSC) on the respective ability to produce reactive oxygen species (ROS). METHODS: The inhibition rates of cell growth in ASC and VSC in co-culture or independent culture was detected after exposed to excitatory stimulus by MTT method. Real-time observation of ROS production by ASC and VSC labeled with Hoechst 33342 was detected by confocal microscopy under the conditions of co-culture or independent culture. RESULTS: Higher concentration of glutamate induced a higher inhibition rate in mixed cell growth than that in ASC alone, while lower concentration of glutamate induced a higher inhibition rate in mixed cell growth than that in VSC only. Real-time observation by confocal microscopy showed that ROS production by VSC under the condition of co-culture, which showed a notable increase at 15 min, was significant less than that in independent culture, which peaked at 5 min and was gradually decreased. ROS production by ASC in co-culture began to increase significantly at 10 min. CONCLUSION: Compared to independent culture, ASC reduces the resting ROS production by co-cultured with VSC, while ASC prolongs the duration of ROS production by VSC after exposed to excitatory stimulus.