生物技术
生物技術
생물기술
BIOTECHNOLOGY
2009年
6期
51-53
,共3页
杨伟超%汲涌%荆士华%张朝印%姜铭妍%谌明%徐慧
楊偉超%伋湧%荊士華%張朝印%薑銘妍%諶明%徐慧
양위초%급용%형사화%장조인%강명연%심명%서혜
2-酮基-L-古龙酸%Vc二步发酵%伴生菌%初筛
2-酮基-L-古龍痠%Vc二步髮酵%伴生菌%初篩
2-동기-L-고룡산%Vc이보발효%반생균%초사
2-keto-L-gulonic acid%two-stage fermentation of vitamin C%associated bacteria%preliminary screening
目的:建立一种Vc二步发酵高效伴生菌的快速初筛方法.方法:通过测定JOD_(650),以伴生菌进入对数期的早晚和对数期持续长短为判断菌株伴生潜力的依据.结果:10h前进入对数期,对数期持续时间约12h的菌株具有高效的促进小菌生长和发酵产古龙酸的潜力.概率为71%.结论:以伴生菌进入对数期的早晚和对数期持续长短为判断依据的新的菌株快速初筛方法,简便、高效,利于大规模初筛应用.
目的:建立一種Vc二步髮酵高效伴生菌的快速初篩方法.方法:通過測定JOD_(650),以伴生菌進入對數期的早晚和對數期持續長短為判斷菌株伴生潛力的依據.結果:10h前進入對數期,對數期持續時間約12h的菌株具有高效的促進小菌生長和髮酵產古龍痠的潛力.概率為71%.結論:以伴生菌進入對數期的早晚和對數期持續長短為判斷依據的新的菌株快速初篩方法,簡便、高效,利于大規模初篩應用.
목적:건립일충Vc이보발효고효반생균적쾌속초사방법.방법:통과측정JOD_(650),이반생균진입대수기적조만화대수기지속장단위판단균주반생잠력적의거.결과:10h전진입대수기,대수기지속시간약12h적균주구유고효적촉진소균생장화발효산고룡산적잠력.개솔위71%.결론:이반생균진입대수기적조만화대수기지속장단위판단의거적신적균주쾌속초사방법,간편、고효,리우대규모초사응용.
Objective: A new preliminary screening method of associated bacteria in two-stage fermentation of vitamin C was established in this article.Method: the judgment basis was, by determining OD_(630) of bacteria, that the time when bacteria entered into log phase and how long the log phase sustained.Result: The screened bacteria whose log phase entered in 10 hours and sustained for 12 hours had a potential to promote the growth of Gluconobacter oxydans, and improve its ability to product L-gulonic acid. the probability was 71 percent. Conclusion: The new preliminary screening method, on the basis of time when bacteria entered into log phase and how long the log phase sustained, was proved effective and easy to operate.
