CAJ | 학술논문

[目的]为对圆口纲动物雷氏七鳃鳗的深入研究及开发利用提供基础的生理生化指标依据.[方法]采用聚丙烯酰胺凝胶垂直板电泳方法对雷氏七鳃鳗肌肉、肠、生殖腺、肝4种组织中乳酸脱氢酶(LDH)和超氧化物歧化酶(SOD)同工酶位点及其酶谱表型进行了研究.[结果]雷氏七鳃鳗不同组织中LDH同工酶呈二带型分布,肌肉和生殖腺中有2条带,而肠和肝中只呈现1条带;不同组织中SOD同工酶的谱带分布特点是:肌肉10条,肠10条,生殖腺9条,肝12条.[结论]SOD和LDH同工酶在雷氏七鳃鳗4种组织中表达存在明显的组织特异性.
[목적]위대원구강동물뢰씨칠새만적심입연구급개발이용제공기출적생리생화지표의거.[방법]채용취병희선알응효수직판전영방법대뢰씨칠새만기육、장、생식선、간4충조직중유산탈경매(LDH)화초양화물기화매(SOD)동공매위점급기매보표형진행료연구.[결과]뢰씨칠새만불동조직중LDH동공매정이대형분포,기육화생식선중유2조대,이장화간중지정현1조대;불동조직중SOD동공매적보대분포특점시:기육10조,장10조,생식선9조,간12조.[결론]SOD화LDH동공매재뢰씨칠새만4충조직중표체존재명현적조직특이성.
[Objective] The study aimed to offer the basal physiological indexes in Lampetra reissneri of Cyclostomata for further research, exploitation and utilization. [Method] Vertical slab polyacrylamide gel electrophoresis method was used to analyze lactate dehydrogenase (LDH) and superoxide dismutase (SOD) isozyme loci and zymogram phenotypes of Lampetra reissneri's four kinds of tissues including muscle, intestine, gonad and liver. [Result] Band distribution of LDH isozymes in different tissues of Lampetra reissneri was double banded. There were two bands in muscle and gonad while only one band was shown in intestine and liver. Band distribution of SOD isozyme in different tissues was characterized by muscle with ten, intestine with ten, gonad with nine, liver with twelve. [Conclusion] SOD and LDH isozymes expressed differently in 4 kinds of tissues of Lampetra reissneri, and there were significant tissue specificities.