肠外与肠内营养
腸外與腸內營養
장외여장내영양
PARENTERAL & ENTERAL NUTRITION
2010年
2期
93-97
,共5页
江海涛%朱维铭%顾立立%曲林林%李秋荣%黎介寿
江海濤%硃維銘%顧立立%麯林林%李鞦榮%黎介壽
강해도%주유명%고립립%곡림림%리추영%려개수
骨髓间充质干细胞移植%缺血-再灌注%肠黏膜通透性
骨髓間充質榦細胞移植%缺血-再灌註%腸黏膜通透性
골수간충질간세포이식%결혈-재관주%장점막통투성
Mesenchymal stem cell%Ischemia/reperfusion%Gut permeability
目的: 观察骨髓间充质干细胞移植对缺血-再灌注损伤的肠黏膜通透性变化的影响. 方法: 体外分离、培养和扩增雄性SD大鼠骨髓间充质干细胞,将雌性SD大鼠随机分为三组,A组为假手术组,仅行剖腹,不夹闭肠系膜上动脉;B组大鼠行肠系膜上动脉夹闭45 min后开放,取培养至第三代的骨髓间充质干细胞直接肠黏膜下注射移植;C组大鼠夹闭肠系膜上动脉45 min后开放,黏膜下注射等量的等渗盐水.术后第3和第6天行乳果糖/甘露醇(L/M)灌胃,6 h后收集尿液测定尿L/M比值.取回肠组织行Y染色体原位杂交检测供鼠来源的细胞.测定血浆D-乳酸水平. 结果: 移植的骨髓间充质干细胞能向缺血-再灌注损伤的肠黏膜归巢,细胞移植的大鼠肠黏膜通透性较C组明显降低(P<0.05). 结论: 骨髓间充质干细胞移植可抑制缺血-再灌注损伤后肠黏膜通透性的升高,维护肠黏膜机械屏障的完整性.
目的: 觀察骨髓間充質榦細胞移植對缺血-再灌註損傷的腸黏膜通透性變化的影響. 方法: 體外分離、培養和擴增雄性SD大鼠骨髓間充質榦細胞,將雌性SD大鼠隨機分為三組,A組為假手術組,僅行剖腹,不夾閉腸繫膜上動脈;B組大鼠行腸繫膜上動脈夾閉45 min後開放,取培養至第三代的骨髓間充質榦細胞直接腸黏膜下註射移植;C組大鼠夾閉腸繫膜上動脈45 min後開放,黏膜下註射等量的等滲鹽水.術後第3和第6天行乳果糖/甘露醇(L/M)灌胃,6 h後收集尿液測定尿L/M比值.取迴腸組織行Y染色體原位雜交檢測供鼠來源的細胞.測定血漿D-乳痠水平. 結果: 移植的骨髓間充質榦細胞能嚮缺血-再灌註損傷的腸黏膜歸巢,細胞移植的大鼠腸黏膜通透性較C組明顯降低(P<0.05). 結論: 骨髓間充質榦細胞移植可抑製缺血-再灌註損傷後腸黏膜通透性的升高,維護腸黏膜機械屏障的完整性.
목적: 관찰골수간충질간세포이식대결혈-재관주손상적장점막통투성변화적영향. 방법: 체외분리、배양화확증웅성SD대서골수간충질간세포,장자성SD대서수궤분위삼조,A조위가수술조,부행부복,불협폐장계막상동맥;B조대서행장계막상동맥협폐45 min후개방,취배양지제삼대적골수간충질간세포직접장점막하주사이식;C조대서협폐장계막상동맥45 min후개방,점막하주사등량적등삼염수.술후제3화제6천행유과당/감로순(L/M)관위,6 h후수집뇨액측정뇨L/M비치.취회장조직행Y염색체원위잡교검측공서래원적세포.측정혈장D-유산수평. 결과: 이식적골수간충질간세포능향결혈-재관주손상적장점막귀소,세포이식적대서장점막통투성교C조명현강저(P<0.05). 결론: 골수간충질간세포이식가억제결혈-재관주손상후장점막통투성적승고,유호장점막궤계병장적완정성.
Objective: To investigate the effect of bone marrow mesenchymal stem cells (MSC) on the variation of intestinal permeability damaged by superior mesenteric artery ischemia and reperfusion. Methods: Bone marrow mesenchymal stem cells were isolated from cavity of tibias and femurs of male Sprague Dawley rat in a sterile condition, and were cultured and proliferated in plastic dishes. 10 week old female Sprague Dawley rats were randomly divided into three groups:group A (sham group), group B (MSC group) and group C (saline group). In group B and group C, the superior mesenteric artery (SMA) of the animals were seperated and occluded by non-invasive vascular clamp for 45 minutes. Immediately after removing the vascular clamp,1×10~7 MSC suspended in 0.5 ml sterile L-DMEM and the same volume of normal saline was submucosally injected into the small intestine at ten different points in group B and group C, respectively. In group A, the animals were only underwent laparotomy without clamping the SMA. 3 days and 6 days after the operation, 100 mg lactulose and 50mg mannitol dissolved in 2 ml distilled water were administrated by oral gavage and urine during 6 h experiment was collected for assaying the L/M ratio before sacrificing the animals. The donor derived MSC was identified by Y chromosome in situ hybridization in ileum tissue, and the serum D-lactate level was determined. Results: The donor derived MSC could home to the ischemia/reperfusion injured intestinal mucosa, and the intestinal permeability was much lower in group B (MSC group) than that in group C (saline group)(P<0.05). Conclusion: Mesenchymal stem cells can reduce the small intestinal mucosal permeability impaired by ischemia/reperfusion, and can participate in the preservation of integrity of the damaged gut mucosal mechanical barrier.