CAJ | 학술논문

目的:探讨不同灸法对溃疡性结肠炎(ulcerativecolitis,UC)大鼠结肠损伤的改善作用,以及对Bcl-2 mRNA、Bax mRNA表达的影响.方法:采用免疫学方法加局部刺激制备实验性UC大鼠模型.造模后采用不同灸法治疗14次,实时荧光定量PCR技术检测结肠组织Bcl-2 mRNA、Bax mRNA表达.结果:(1)各治疗组结肠大体形态损伤积分和光镜下组织学损伤积分较模型组比较有明显改善(P＜0.05,P＜0.01);(2)UC模型大鼠结肠组织Bcl-2 mRNA的表达显著高于正常大鼠(P＜0.01),而Bax mRNA的表达显著低于正常大鼠(P＜0.01);(3)治疗后,隔药灸组、隔姜灸组、隔蒜灸组大鼠结肠组织Bcl-2 mRNA的表达明显下调,与模型组比较有显著差异(P＜0.05或P＜0.01);隔药灸组、隔姜灸组Bax mRNA的表达明显增高,与模型组比较有显著差异(P＜0.05).结论:初步研究结果提示隔药灸、隔姜灸、隔蒜灸、温和灸治疗均能有效改善UC大鼠结肠病理损伤,隔药灸、隔姜灸、隔蒜灸可不同程度抑制UC大鼠结肠组织Bcl-2 mRNA和Bax mRNA的表达,调节Bcl-2/Bax mRNA的比例关系.
목적:탐토불동구법대궤양성결장염(ulcerativecolitis,UC)대서결장손상적개선작용,이급대Bcl-2 mRNA、Bax mRNA표체적영향.방법:채용면역학방법가국부자격제비실험성UC대서모형.조모후채용불동구법치료14차,실시형광정량PCR기술검측결장조직Bcl-2 mRNA、Bax mRNA표체.결과:(1)각치료조결장대체형태손상적분화광경하조직학손상적분교모형조비교유명현개선(P＜0.05,P＜0.01);(2)UC모형대서결장조직Bcl-2 mRNA적표체현저고우정상대서(P＜0.01),이Bax mRNA적표체현저저우정상대서(P＜0.01);(3)치료후,격약구조、격강구조、격산구조대서결장조직Bcl-2 mRNA적표체명현하조,여모형조비교유현저차이(P＜0.05혹P＜0.01);격약구조、격강구조Bax mRNA적표체명현증고,여모형조비교유현저차이(P＜0.05).결론:초보연구결과제시격약구、격강구、격산구、온화구치료균능유효개선UC대서결장병리손상,격약구、격강구、격산구가불동정도억제UC대서결장조직Bcl-2 mRNA화Bax mRNA적표체,조절Bcl-2/Bax mRNA적비례관계.
Objective: To investigate the improving effects of different moxibustion therapies on colonic injury in rats with ulcerative colitis (UC), and on the expression of Bcl-2 and Bax mRNA.Methods: Rats model of UC was made with immune methods and local stimulation. The models were treated with different moxibustion therapies 14 times, and then the expression of Bcl-2 and Bax mRNA were tested in the colonic tissue with fluorescent quantitative PCR. Results: (1)General scores of colon and histologic scores of injury were improved in all treatment groups except the model group (P＜0.05, P＜0.01); (2) The expression of Bcl-2 mRNA in the model rats of UC was higher than that in the normal rats (P＜0.01), whereas Bax lower (P＜0.01); (3) After treatments, the expression of Bcl-2 mRNA was lower in the herb cake-partitioned moxibustion, ginger-partitioned moxibustion, and garlic-partitioned moxibustion groups than that in the model group (P＜0.05, P＜0.01), and Bax mRNA in the herb cake-partitioned and ginger-partitioned groups was higher (P＜0.05). Conclusion: Herb cake-partitioned moxibustion, ginger-partitioned moxibustion, garlic-partitioned moxibustion, and mild moxibustion could improve the pathological injury in UC rats. Herb cake-partitioned moxibustion, ginger-partitioned moxibustion, and garlic-partitioned moxibustion could inhibit the expression of Bcl-2 mRNA and Bax mRNA in the colonic tissue of UC rats to regulate the ratio of Bcl-2/Bax mRNA.