CAJ | 학술논문

[目的]优化南方红豆杉愈伤组织诱导及训化条件.[方法]以南方红豆杉幼芽、幼茎以及幼叶为外植体,比较不同外植体诱导愈伤组织的效率,并对愈伤组织的增殖条件及悬浮细胞培养条件进行了研究.[结果]以幼芽作为外植体的诱导效果最佳;南方红豆杉外植体消毒的最佧方法为链霉索浸泡2h+洗沽精浸泡3h+75%酒精消毒30s+10%次氯酸钠浸泡25min+1‰氯化汞10 min;愈伤继代的最佳配方为B5+4.0 mg/L NAA+O.5 mg/L 2.4-D+O.2mg/L GA+0.5mg/L6-BA+2g/L活性碳.[结论]该研究建立了南方红豆杉的高效再生体系.
[목적]우화남방홍두삼유상조직유도급훈화조건.[방법]이남방홍두삼유아、유경이급유협위외식체,비교불동외식체유도유상조직적효솔,병대유상조직적증식조건급현부세포배양조건진행료연구.[결과]이유아작위외식체적유도효과최가;남방홍두삼외식체소독적최카방법위련매색침포2h+세고정침포3h+75%주정소독30s+10%차록산납침포25min+1‰록화홍10 min;유상계대적최가배방위B5+4.0 mg/L NAA+O.5 mg/L 2.4-D+O.2mg/L GA+0.5mg/L6-BA+2g/L활성탄.[결론]해연구건립료남방홍두삼적고효재생체계.
[Objective]The paper aims to optimize the abduction and domestication conditions of Taxus chinensis var.Mairei callus.[Method]We compared the efficiencies of callus between different explants and investigated the multiplication conditions of callus and suspended cell culture conditions with the buds,young stems and young leaves from T.chinensis vat as the explants.[Results]The effect was the best with the bud as the explants; the best way for sterilizing the explants of T.chinensis var mairei was:streptomycin detergent for 2 h+suds for 3 h+75% alcohol disinfection for 30 s+10% sodium hypochlorite solution for 25 min+1‰ mercury chloride for 10 min; the optimum formula of callus subculture was:B5+4.0 mg/L NAA+0.5 mg/L2,4-D+0.2 mg/L GA+0.5 mg/L6-BA+2 g/L AC.[Conclusion]This research built the high efficient regeneration system of T.chinensis var.