中国地方病学杂志
中國地方病學雜誌
중국지방병학잡지
CHINESE JOURNAL OF ENDEMIOLOGY
2009年
4期
361-364
,共4页
周青%苏丽琼%李蓓%苏鹏%郑克纯%韦蝶心%宋志忠
週青%囌麗瓊%李蓓%囌鵬%鄭剋純%韋蝶心%宋誌忠
주청%소려경%리배%소붕%정극순%위접심%송지충
耶尔森菌,鼠疫%蛋白芯片%血清%抗体
耶爾森菌,鼠疫%蛋白芯片%血清%抗體
야이삼균,서역%단백심편%혈청%항체
Yersinia pestis%Protein microarray%blood serum%Antibody
目的 分析肺鼠疫患者血清中蛋白抗体产生的种类和机体免疫应答能力,为筛选新的疫苗亚单位提供试验依据.方法 利用蛋白微阵列技术构建蛋白芯片.对2例肺鼠疫患者发病后6个月的血清,采用蛋白质芯片(含有149个鼠疫菌毒力相关基因蛋白)技术检测血清中鼠疫蛋白抗体的种类和机体的免疫应答能力.结果 患者1血清中除了YPMT1.23c、YPMT1.86、YP00406、YP01071基因编码的蛋白没检测出相应的蛋白抗体外,其他88种蛋白均检测出相应的蛋白抗体;患者2血清中检测出43种基因编码的蛋白抗体,其他49种没有检查到.有39种基因蛋白抗体在患者1、2血清中都存在,其中有YPMT1.81c、YPMT1.84、rv10、YPCD1.31c、YPCD1.28、YPCD1.58、YPMT1.62c、YP03247共8种蛋白抗体明显升高.与正常值比较,患者1、2血清中8种蛋白抗体升高的阳性倍数分别为:109.96、176.4,20.64、17.73,16.50、7.16,23.51、7.65,46.00、25.61,4.50、8.24,5.98、5.08,23.98、4.76.结论 通过对肺鼠疫患者血清抗体测定,8种蛋白抗体不仅可作为免疫诊断的靶标,同时也是今后亚单位疫苗的研究重点.
目的 分析肺鼠疫患者血清中蛋白抗體產生的種類和機體免疫應答能力,為篩選新的疫苗亞單位提供試驗依據.方法 利用蛋白微陣列技術構建蛋白芯片.對2例肺鼠疫患者髮病後6箇月的血清,採用蛋白質芯片(含有149箇鼠疫菌毒力相關基因蛋白)技術檢測血清中鼠疫蛋白抗體的種類和機體的免疫應答能力.結果 患者1血清中除瞭YPMT1.23c、YPMT1.86、YP00406、YP01071基因編碼的蛋白沒檢測齣相應的蛋白抗體外,其他88種蛋白均檢測齣相應的蛋白抗體;患者2血清中檢測齣43種基因編碼的蛋白抗體,其他49種沒有檢查到.有39種基因蛋白抗體在患者1、2血清中都存在,其中有YPMT1.81c、YPMT1.84、rv10、YPCD1.31c、YPCD1.28、YPCD1.58、YPMT1.62c、YP03247共8種蛋白抗體明顯升高.與正常值比較,患者1、2血清中8種蛋白抗體升高的暘性倍數分彆為:109.96、176.4,20.64、17.73,16.50、7.16,23.51、7.65,46.00、25.61,4.50、8.24,5.98、5.08,23.98、4.76.結論 通過對肺鼠疫患者血清抗體測定,8種蛋白抗體不僅可作為免疫診斷的靶標,同時也是今後亞單位疫苗的研究重點.
목적 분석폐서역환자혈청중단백항체산생적충류화궤체면역응답능력,위사선신적역묘아단위제공시험의거.방법 이용단백미진렬기술구건단백심편.대2례폐서역환자발병후6개월적혈청,채용단백질심편(함유149개서역균독력상관기인단백)기술검측혈청중서역단백항체적충류화궤체적면역응답능력.결과 환자1혈청중제료YPMT1.23c、YPMT1.86、YP00406、YP01071기인편마적단백몰검측출상응적단백항체외,기타88충단백균검측출상응적단백항체;환자2혈청중검측출43충기인편마적단백항체,기타49충몰유검사도.유39충기인단백항체재환자1、2혈청중도존재,기중유YPMT1.81c、YPMT1.84、rv10、YPCD1.31c、YPCD1.28、YPCD1.58、YPMT1.62c、YP03247공8충단백항체명현승고.여정상치비교,환자1、2혈청중8충단백항체승고적양성배수분별위:109.96、176.4,20.64、17.73,16.50、7.16,23.51、7.65,46.00、25.61,4.50、8.24,5.98、5.08,23.98、4.76.결론 통과대폐서역환자혈청항체측정,8충단백항체불부가작위면역진단적파표,동시야시금후아단위역묘적연구중점.
Objective To analyze the species of the antibody and immune responsibility in pneumonic plague patients in order to pave the way to screen the new sub-unit of the vaccine to provide the experimental basis. Methods Using the virulence-related protein microarray containing 149 proteins of Yersinia pestis (Y.pestis), the species of the antibody and immune responsibility were analyzed in serum of two pneumonic plague patients in six months after onset. Results Eighty-eight gene coded proteins were detected out the related antibodies except YPMT1.23c, YPMT1.86, YPO0406 and YPO1071 in patient 1. Forty-three antibodies from gene coded protein were analyzed, other forty-nine had not been identified in patient 2. Thirty-nine antibodies were detected in both patients. The proteins YPMT1.81c, YPMT1.84, YPCD1.31c, rw10, YPCD1.28, YPCD1.58, YPMT1.62c, YPO3247-related antibodies increased significantly by 109.96,176.4 ;20.64,17.73 ;16.50,7.16 ;23.51,7.65 ;46.00,25.61 ;4.50,8.24 ;5.98,5.08 ;23.98,4.76 folds, respectively. Conclusions The study on the antibody in pneumonic plague patients helps us to select the potential vaccine candidates, which reveals that eight proteins are the immunity diagnosis targets and the research key of sub-unit vaccine.
